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Literature summary for 2.1.1.41 extracted from
- Mechanism and inhibition of delta 24-sterol methyltransferase from Candida albicans and Candida tropicalis (1989), Biochemistry, 28, 9633-9640.
General Stability
| General Stability | Organism |
|---|---|
| no stabilization by MgCl2, CaCl2, DTT, reduced glutathione, phosphatidylcholine | Candida albicans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| desmosterol | competitive versus sterol and S-adenosyl-L-methionine | Candida albicans |  |
| desmosterol | competitive versus sterol and S-adenosyl-L-methionine | Candida tropicalis | |
| S-adenosyl-L-homocysteine | - |
Candida albicans | |
| Substrate analogues | dead-end inhibitors | Candida albicans | |
| Zymosterol | competitive versus sterol and S-adenosyl-L-methionine | Candida albicans | |
| Zymosterol | competitive versus sterol and S-adenosyl-L-methionine | Candida tropicalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.052 | - |
Zymosterol | - |
Candida tropicalis | |
| 0.071 | - |
Zymosterol | - |
Candida albicans | |
| 0.236 | - |
desmosterol | - |
Candida tropicalis | |
| 0.281 | - |
desmosterol | - |
Candida albicans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Candida albicans | - |
- |
| microsome | - |
Candida tropicalis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Candida albicans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| zymosterol + S-adenosyl-L-methionine | Candida albicans | - |
fecosterol + S-adenosyl-L-homocysteine | - |
? | |
| zymosterol + S-adenosyl-L-methionine | Candida tropicalis | - |
fecosterol + S-adenosyl-L-homocysteine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
| Candida tropicalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Candida albicans |
- |
Candida tropicalis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | mechanism | Candida albicans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 1 month, less than 10% loss of activity | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| desmosterol + S-adenosyl-L-methionine | - |
Candida albicans | 24-methylenedesmosterol + S-adenosyl-L-homocysteine | - |
? | |
| desmosterol + S-adenosyl-L-methionine | - |
Candida tropicalis | 24-methylenedesmosterol + S-adenosyl-L-homocysteine | - |
? | |
| lanosterol + S-adenosyl-L-methionine | no reaction with | Candida albicans | 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine | - |
? | |
| zymosterol + S-adenosyl-L-methionine | - |
Candida albicans | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
| zymosterol + S-adenosyl-L-methionine | - |
Candida tropicalis | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
| zymosterol + S-adenosyl-L-methionine | zymosterol is 5alpha-cholesta-8,24-dien-3beta-ol | Candida albicans | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Candida albicans |
| 30 | - |
assay at | Candida tropicalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Candida albicans |
| 7.5 | - |
assay at | Candida tropicalis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0045 | - |
Zymosterol | versus S-adenosyl-L-methionine | Candida tropicalis | |
| 0.009 | - |
desmosterol | versus S-adenosyl-L-methionine | Candida tropicalis | |
| 0.0193 | - |
desmosterol | versus S-adenosyl-L-methionine | Candida albicans | |
| 0.022 | - |
Zymosterol | versus sterol | Candida tropicalis | |
| 0.449 | - |
desmosterol | versus sterol | Candida tropicalis | |
| 0.489 | - |
desmosterol | versus sterol | Candida albicans | |
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