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Literature summary for 2.1.1.41 extracted from
- Isolation and purification of an S-adenosylmethionine: delta 24-sterol methyltransferase from yeast (1969), J. Biol. Chem., 244, 6334-6340.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | required | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
Zymosterol | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, required | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| zymosterol + S-adenosyl-L-methionine | Saccharomyces cerevisiae | - |
fecosterol + S-adenosyl-L-homocysteine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity at various concentrations of glutathione | Saccharomyces cerevisiae |
| 0.53 | - |
purified enzyme | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.1 M Tris-HCl buffer, 1 mM Mg2+, 90% loss of activity overnight | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| zymosterol + S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
| zymosterol + S-adenosyl-L-methionine | zymosterol is 5alpha-cholesta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | fecosterol + S-adenosyl-L-homocysteine | fecosterol is 24-exomethylene-5alpha-cholesta-8,24-dien-3beta-ol | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.7 | less than half-maximal activity above and below | Saccharomyces cerevisiae |
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