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Literature summary for 2.1.1.38 extracted from

  • Rao, M.M.; Rebello, P.F.; Pogell, B.M.
    Biosynthesis of puromycin in Streptomyces alboniger. Enzymatic methylation of O-demethylpuromycin (1969), J. Biol. Chem., 244, 112-118.
    View publication on PubMed

Application

Application Comment Organism
medicine product puromycin is an antibiotic and antitumor agent Streptomyces alboniger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
S-adenosyl-L-methionine
-
Streptomyces alboniger
0.21
-
O-demethylpuromycin
-
Streptomyces alboniger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
sucrose density gradient centrifugation Streptomyces alboniger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + O-demethylpuromycin Streptomyces alboniger
-
S-adenosyl-L-homocysteine + puromycin
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces alboniger
-
-
-

Storage Stability

Storage Stability Organism
-65°C, can be stored for several months without loss of activity Streptomyces alboniger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information L-tyrosine, L-tyrosine ethyl ester, L-tyrosine amide, catechol and epinephrine are not substrates Streptomyces alboniger ?
-
?
S-adenosyl-L-methionine + O-demethylpuromycin
-
Streptomyces alboniger S-adenosyl-L-homocysteine + puromycin
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Streptomyces alboniger
8
-
broad pH optimum over the range 7.0-9.0 with a maximum at pH 8.0 Streptomyces alboniger

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Streptomyces alboniger