Application | Comment | Organism |
---|---|---|
biotechnology | using a truncated and highly active form of human DNMT1 and a designed hemimethylated DNA substrate, a robust, efficient, and economical fluorescence assay is developped suitable for in vitro high-throughput screening of DNMT1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELAT1-501 | to accelerate the rate of DNMT1 with the hemimethylated substrate, the 501 amino acids at the N terminus are deleted. Truncated DNMT1 exhibits a 4fold increased kcat/Km as compared with the full-length enzyme and does not require allosteric activation by hemimethylated DNA | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | a hemimethylated 5MeCpG target site is generated using Sau3AI restriction enzyme | Homo sapiens | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dnmt1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay carried out at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |