Literature summary for 2.1.1.37 extracted from

Teerawanichpan, P.; Krittanai, P.; Chauvatcharin, N.; Narangajavana, J.
Purification and characterization of rice DNA methyltransferase (2009), Plant Physiol. Biochem., 47, 671-680.

Search for more literature for 2.1.1.37

Cloned(Commentary)

Cloned (Comment)	Organism
gene MET1-1, DNA and amino acid sequence determination and analysis	Oryza sativa
gene MET1-2, DNA and amino acid sequence determination and analysis	Oryza sativa

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	inhibitory effects by methyl donor analogs, base analogs, cations, and cationic amines on rice DNA MTase; inhibitory effects by methyl donor analogs, base analogs, cations, and cationic amines on rice DNA MTase	Oryza sativa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Oryza sativa
0.00263	-	S-adenosyl-L-methionine	pH 7.6, 25°C	Oryza sativa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	DNA MTase is enriched in nuclei	Oryza sativa	5634	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
160000	170000	gel filtration	Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA + S-adenosyl-L-methionine	Oryza sativa	-	DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	B1Q3J6	MET1-2; genes MET1-2	-
Oryza sativa	Q7Y1I7	MET1-1; gene MET1-1	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from nuclear extracts from shoots of 10-day-old rice seedlings, 800fold by anion exchange and heparin affinity chromatography, and gel filtration	Oryza sativa

Source Tissue

Source Tissue	Comment	Organism	Textmining
callus	-	Oryza sativa	-
inflorescence	-	Oryza sativa	-
leaf	only the OsMET1-1 mRNAis slightly accumulated in young leaves, in which virtually no OsMET1-2 transcripts is detectable	Oryza sativa	-
additional information	only the OsMET1-1 mRNAis slightly accumulated in young leaves, in which virtually no OsMET1-2 transcripts is detectable	Oryza sativa	-
root	young	Oryza sativa	-
seed	DNA MTase activity is remarkably increased during imbibing dry seeds	Oryza sativa	-
seedling	-	Oryza sativa	-
shoot	-	Oryza sativa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0013	-	purified enzyme with substrate hemi-methylated DNA substrate poly(dI-mdC)poly(dI-dC)	Oryza sativa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + S-adenosyl-L-methionine	-	Oryza sativa	DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine	-	?
DNA + S-adenosyl-L-methionine	substrate is chloroplast DNA, no activity with mitochondrial DNA. Substrate sources are etiolated leaf-rice DNA, dry embryo-rice DNA, 10-day-old shoot-rice DNA, mature leaf-rice DNA. The purified protein prefers hemi-methylated DNA substrate, substrate specificity, the enzyme is specific for d(C-G) sites, overview	Oryza sativa	DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine	-	?
poly(dG-mdC)-poly(dG-dC) + S-adenosyl-L-methionine	-	Oryza sativa	?	-	?
poly(dI-mdC)-poly(dI-dC) + S-adenosyl-L-methionine	-	Oryza sativa	?	-	?

Synonyms

Synonyms	Comment	Organism
DNA methyltransferase	-	Oryza sativa
DNA MTase	-	Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Oryza sativa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	8	-	Oryza sativa

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Oryza sativa

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Release 2023.1 (January 2023)