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Literature summary for 2.1.1.37 extracted from

  • Chiou, C.S.; Li, H.Y.; Tung, S.K.; Chen, C.Y.; Teng, C.H.; Shu, J.C.; Tseng, J.T.; Hsu, C.Y.; Chen, C.C.
    Identification of prophage gene z2389 in Escherichia coli EDL933 encoding a DNA cytosine methyltransferase for full protection of NotI sites (2010), Int. J. Med. Microbiol., 300, 296-303.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene z2389, located within the genome of prophage CP-933R, sequence comparisonsn, expression of His6-tagged enzyme under the control of an arabinose-inducible promoter in Escherichia coli strain BW25113 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of a z2389 null mutant Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + S-adenosyl-L-methionine Escherichia coli the Z2389 DNAcytosine methyltransferase confers full protection to NotI sites by methylation of the first cytosine residue DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
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?

Organism

Organism UniProt Comment Textmining
Escherichia coli Q8X8S5 strain EDL933, a O157:H7 strain, gene z2389, which is located within the genome of prophage CP-933R
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BW25113 by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + S-adenosyl-L-methionine the Z2389 DNAcytosine methyltransferase confers full protection to NotI sites by methylation of the first cytosine residue Escherichia coli DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
?
DNA + S-adenosyl-L-methionine Z2389 methylates NotI site , i.e. GCGGCCGC, and EagI site, both at the first cytosine residue Escherichia coli DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
?

Synonyms

Synonyms Comment Organism
DNA cytosine methyltransferase
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Escherichia coli
Z2389
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Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Escherichia coli

General Information

General Information Comment Organism
physiological function epigenetic methylation is the most common DNA modification in prokaryotes and eukaryotes and is carried out by DNA methyltransferases Escherichia coli