Cloned (Comment) | Organism |
---|---|
gene z2389, located within the genome of prophage CP-933R, sequence comparisonsn, expression of His6-tagged enzyme under the control of an arabinose-inducible promoter in Escherichia coli strain BW25113 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a z2389 null mutant | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | Escherichia coli | the Z2389 DNAcytosine methyltransferase confers full protection to NotI sites by methylation of the first cytosine residue | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q8X8S5 | strain EDL933, a O157:H7 strain, gene z2389, which is located within the genome of prophage CP-933R | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BW25113 by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | the Z2389 DNAcytosine methyltransferase confers full protection to NotI sites by methylation of the first cytosine residue | Escherichia coli | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? | |
DNA + S-adenosyl-L-methionine | Z2389 methylates NotI site , i.e. GCGGCCGC, and EagI site, both at the first cytosine residue | Escherichia coli | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA cytosine methyltransferase | - |
Escherichia coli |
Z2389 | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | epigenetic methylation is the most common DNA modification in prokaryotes and eukaryotes and is carried out by DNA methyltransferases | Escherichia coli |