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Literature summary for 2.1.1.37 extracted from

  • Darii, M.V.; Cherepanova, N.A.; Subach, O.M.; Kirsanova, O.V.; Rasko, T.; Slaska-Kiss, K.; Kiss, A.; Deville-Bonne, D.; Reboud-Ravaux, M.; Gromova, E.S.
    Mutational analysis of the CG recognizing DNA methyltransferase SssI: insight into enzyme-DNA interactions (2009), Biochim. Biophys. Acta, 1794, 1654-1662.
    View publication on PubMed
Search for more literature for 2.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged SssI in Escherichia coli strain ER1821 Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
C141S site-directed mutagenesis in motif IV Synechocystis sp.
C368A the mutation does not affect the enzyme activity Synechocystis sp.
E186A site-directed mutagenesis in motif VI, almost inactive mutant Synechocystis sp.
K297A site-directed mutagenesis in motif TRD, the mutant is resistant to Hin6I digestion Synechocystis sp.
N299A site-directed mutagenesis in motif TRD, the mutant is resistant to Hin6I digestion Synechocystis sp.
Q147L site-directed mutagenesis in motif IV, the mutant shows less than 10% of wild-type activity Synechocystis sp.
R230A site-directed mutagenesis in motif VIII, almost inactive mutant Synechocystis sp.
R232A site-directed mutagenesis in motif VIII, the mutant shows less than 10% of wild-type activity Synechocystis sp.
S145A site-directed mutagenesis in motif IV, the mutant shows less than 10% of wild-type activity Synechocystis sp.
S2C/C141S/C368A replacement of the active site Cys141 reduces the enzyme activity, while the substitutions Ser2Cys and Cys368Ala as well as a C-terminal Ser-His6 tag do not affect the enzyme activity Synechocystis sp.
S317A site-directed mutagenesis in motif TRD, the mutant shows about 50% of wild-type activity Synechocystis sp.
T313A site-directed mutagenesis in motif TRD, the mutant is resistant to Hin6I digestion Synechocystis sp.
T313D site-directed mutagenesis in motif TRD, almost inactive mutant Synechocystis sp.
T313H site-directed mutagenesis in motif TRD, almost inactive mutant Synechocystis sp.
V188X replacement of the conserved Val188 from the ENV motif VI results in a 5fold increased DNA binding affinity and a 2fold decreased catalytic activity Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone
-
 Synechocystis sp.
oligodeoxyribonucleotides containing 2-aminopurine
-
 Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information recombinant kinetics of wild-type and mutant enzymes, overview Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + S-adenosyl-L-methionine Synechocystis sp. M.SssI is the only known prokaryotic C5-MTase, which recognizes the short sequence CG and thus has the same specificity as mammalian MTases DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged SssI from Escherichia coli strain ER1821 by cobalt affinity chromatography Synechocystis sp.

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + DNA containing cytosine = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine catalytic reaction mechanism Synechocystis sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + S-adenosyl-L-methionine M.SssI is the only known prokaryotic C5-MTase, which recognizes the short sequence CG and thus has the same specificity as mammalian MTases Synechocystis sp. DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?
DNA + S-adenosyl-L-methionine structural basis for DNA recognition and base flipping, overview Synechocystis sp. DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?

Synonyms

Synonyms Comment Organism
C5-MTase
-
 Synechocystis sp.
CG recognizing DNA methyltransferase SssI
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Synechocystis sp.