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Literature summary for 2.1.1.37 extracted from
- Purification and properties of a novel DNA methyltransferase from cultured rice cells (1991), Biochem. Biophys. Res. Commun., 177, 711-719.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value for poly(dI-MedC)*poly(dI-dC): 0.017 mg/ml | Oryza sativa | |
| 0.0026 | - |
S-adenosyl-L-methionine | - |
Oryza sativa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
sucrose density gradient centrifugation | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
L. cv Roncarolo | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryza sativa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for more than 2 months | Oryza sativa |
| 4°C, stable for 5 days | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA | - |
Oryza sativa | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
| S-adenosyl-L-methionine + DNA | native plant DNA or hemimethylated poly(dI-MedC)*poly(dI-dC) | Oryza sativa | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
stable for 5 days | Oryza sativa |
| 30 | - |
15 min, stable | Oryza sativa |
| 42 | - |
half-life: 48 s | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8.7 | 50% of maximal activity at pH 7.6 and at pH 8.7 | Oryza sativa |
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Release 2023.1 (January 2023)
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