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Literature summary for 2.1.1.37 extracted from

  • Simon, D.; Grunert, F.; v.Acken, U.; Döring, H.P.; Kröger, H.
    DNA-methylase from regenerating rat liver: purification and characterisation (1978), Nucleic Acids Res., 5, 2153-2167.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.37

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine potent competitive inhibitor Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 S-adenosyl-L-methionine in presence of Micrococcus luteus DNA Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Rattus norvegicus 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 glycerol gradient centrifugation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-70°C, the purified enzyme preparation is stable for at least 6 months Rattus norvegicus
4°C, the purified enzyme preparation is rather unstable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA
-
 Rattus norvegicus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
S-adenosyl-L-methionine + DNA the enzyme is able to methylate single stranded and double stranded DNA. Previously unmethylated double-stranded DNA from prokaryotes, Micrococcus luteus, as well as from eukaryotes, Ascaris suis, can serve as substrates. The synthetic copolymers (dG-dC)n*(dC-dG)n and (dG,dC)n are also methylated. SV40 DNA is almost not methylated. PM2 DNA is a good substrate even in the supercoiled form. The enzyme methylates 1 in 17 bases in heterologous Micrococcus luteus DNA, but only 1 in 590 in homologous rat liver DNA Rattus norvegicus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00033
-
 S-adenosyl-L-homocysteine
-
 Rattus norvegicus