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Literature summary for 2.1.1.361 extracted from

  • Fang, J.; Feng, Q.; Ketel, C.S.; Wang, H.; Cao, R.; Xia, L.; Erdjument-Bromage, H.; Tempst, P.; Simon, J.A.; Zhang, Y.
    Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase (2002), Curr. Biol., 12, 1086-1099 .
    View publication on PubMed
Search for more literature for 2.1.1.361

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
H299A the mutation greatly impairs the enzymatic activity toward nucleosomal histone H4 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleosome
-
 Homo sapiens 786
-
nucleosome
-
 Drosophila melanogaster 786
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000 150000 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is completely inactive toward histone H4 with a K20L mutation ?
-
 ?
additional information Drosophila melanogaster the enzyme is completely inactive toward histone H4 with a K20L mutation ?
-
 ?
S-adenosyl-L-methionine + a [histone H4]-L-lysine20 Homo sapiens highly specific substrate S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?
S-adenosyl-L-methionine + a [histone H4]-L-lysine20 Drosophila melanogaster highly specific substrate S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9VFK6
-
-
Homo sapiens Q9NQR1
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE52 column chromatography, phosphocellulose P11 column chromatography, DEAE-5PW column chromatography, and hydroxyapatite column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is completely inactive toward histone H4 with a K20L mutation Homo sapiens ?
-
 ?
additional information the enzyme is completely inactive toward histone H4 with a K20L mutation Drosophila melanogaster ?
-
 ?
S-adenosyl-L-methionine + a [histone H4]-L-lysine20 highly specific substrate Homo sapiens S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?
S-adenosyl-L-methionine + a [histone H4]-L-lysine20 highly specific substrate Drosophila melanogaster S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
CG3307
-
 Drosophila melanogaster
H4-K20-specific HMTase
-
 Homo sapiens
H4-K20-specific HMTase
-
 Drosophila melanogaster
H4-K20-specific methyltransferase
-
 Homo sapiens
H4-K20-specific methyltransferase
-
 Drosophila melanogaster
histone H4-lysine 20-specific methyltransferase
-
 Homo sapiens
histone H4-lysine 20-specific methyltransferase
-
 Drosophila melanogaster
SET8
-
 Homo sapiens
SET8
-
 Drosophila melanogaster

General Information

General Information Comment Organism
malfunction enzyme gene disruption results in lethality Drosophila melanogaster
physiological function the enzyme is essential for Drosophila development Drosophila melanogaster