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Literature summary for 2.1.1.355 extracted from

  • Akoury, E.; Ma, G.; Demolin, S.; Broenner, C.; Zocco, M.; Cirilo, A.; Ivic, N.; Halic, M.
    Disordered region of H3K9 methyltransferase Clr4 binds the nucleosome and contributes to its activity (2019), Nucleic Acids Res., 47, 6726-6736 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.355

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
RNA the presence of RNA strongly inhibits enzyme activity in vitro Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM used in assay conditions Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 Schizosaccharomyces pombe overall reaction 3 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + [histone H3]-L-lysine9 Schizosaccharomyces pombe
-
 S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine9 Schizosaccharomyces pombe
-
 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine9 Schizosaccharomyces pombe
-
 S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine9
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 overall reaction Schizosaccharomyces pombe 3 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + [histone H3]-L-lysine9
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine9
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine9
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine9
-
 ?

Subunits

Subunits Comment Organism
? x * 55920, calculated from amino acid sequence Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
Clr4
-
 Schizosaccharomyces pombe
H3K9 methyltransferase
-
 Schizosaccharomyces pombe

General Information

General Information Comment Organism
physiological function the enzyme contributes to heterochromatin formation Schizosaccharomyces pombe