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Literature summary for 2.1.1.355 extracted from

  • Jang, S.; Kang, C.; Yang, H.S.; Jung, T.; Hebert, H.; Chung, K.Y.; Kim, S.J.; Hohng, S.; Song, J.J.
    Structural basis of recognition and destabilization of the histone H2B ubiquitinated nucleosome by the DOT1L histone H3 Lys79 methyltransferase (2019), Genes Dev., 33, 620-625.
    View publication on PubMed
Search for more literature for 2.1.1.355

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme activity is stimulated by histone H2B Lys120 ubiquitination Homo sapiens

Protein Variants

Protein Variants Comment Organism
F326A the mutant shows significantly reduced activity compared to the wild type enzyme Homo sapiens
F326A the mutation slightly decreases the enzyme activity Homo sapiens
I290A the mutant shows significantly reduced activity compared to the wild type enzyme Homo sapiens
L284A the mutant shows significantly reduced activity compared to the wild type enzyme Homo sapiens
L329A the mutant shows significantly reduced activity compared to the wild type enzyme Homo sapiens
R282A the mutation dramatically decreases the enzyme activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 Homo sapiens overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + a [histone H3]-L-lysine9 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine9 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine9 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + H2B ubiquitinated nucleosome Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8TEK3
-
-

Purification (Commentary)

Purification (Comment) Organism
glutahione S-transferase affinity column chromatography, anion exchange column chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 overall reaction Homo sapiens 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + a [histone H3]-L-lysine9
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine9
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine9
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine9
-
 ?
S-adenosyl-L-methionine + H2B ubiquitinated nucleosome
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
DOT1L
-
 Homo sapiens
histone H3 Lys79 methyltransferase
-
 Homo sapiens