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Literature summary for 2.1.1.355 extracted from

  • Rathert, P.; Zhang, X.; Freund, C.; Cheng, X.; Jeltsch, A.
    Analysis of the substrate specificity of the Dim-5 histone lysine methyltransferase using peptide arrays (2008), Chem. Biol., 15, 5-11.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain XL1 blue Neurospora crassa

Protein Variants

Protein Variants Comment Organism
D209A in solution experiments with the wild type histone H3 sequence, the mutant shows a 100fold reduced activity as compared to wild-type Dim-5 Neurospora crassa
D209S in solution experiments with the wild type histone H3 sequence, the mutant shows a 100fold reduced activity as compared to wild-type Dim-5 Neurospora crassa
E227A in solution experiments with the wild type histone H3 sequence, the mutant shows a 10fold reduced activity as compared to wild-type Dim-5 Neurospora crassa
E227S in solution experiments with the wild type histone H3 sequence, the mutant shows a 10fold reduced activity as compared to wild-type Dim-5 Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012 0.0013 S-adenosyl-L-methionine in methylation buffer containing 50 mM glycine (pH 9.8), 2 mM dithiothreitol, 0.025 mg/ml bovine serum albumin, 10% (v/v) glycerol, at 22°C Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + histone H3(K9) Dim-5 recognizes R8-G12 of the H3 tail with T11 and G12 being the most important specificity determinants, phosphorylation of H3 tail residues S10 and T11may regulate the activity of Dim-5 Neurospora crassa ?
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 ?

Synonyms

Synonyms Comment Organism
Dim-5
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 Neurospora crassa
Dim-5 histone-3 lysine-9 methyltransferase
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 Neurospora crassa
HKMT
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 Neurospora crassa