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Literature summary for 2.1.1.355 extracted from

  • Gowher, H.; Zhang, X.; Cheng, X.; Jeltsch, A.
    Avidin plate assay system for enzymatic characterization of a histone lysine methyltransferase (2005), Anal. Biochem., 342, 287-291.
    View publication on PubMed
Search for more literature for 2.1.1.355

Application

Application Comment Organism
analysis development of a microplate biotin/avidin peptide methylation assay, which is convenient, very accurate, reproducible, and inexpensive. Because it yields quantitative results, it can be employed for a characterization of the enzymatic properties of histone lysine methyltransferases and other protein methyltransferases and is also well suited for high-throughput applications Neurospora crassa

Protein Variants

Protein Variants Comment Organism
F281W shows catalytic activity Neurospora crassa
F281Y shows catalytic activity Neurospora crassa
Y178F shows catalytic activity, although the methylation rates of this variant are low Neurospora crassa
Y178V shows catalytic activity Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0074
-
 biotinyl-MARTKQTARKSTGGKAPRKQ
-
 Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purity greater than 95% Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biotinyl-MARTKQTARKSTGGKAPRKQ + S-adenosyl-L-methionine Dim-5 methylates the histone H3 tail at position Lys9 Neurospora crassa ?
-
 ?

Synonyms

Synonyms Comment Organism
Dim-5
-
 Neurospora crassa
Dim-5 histone lysine methyltransferase
-
 Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
 biotinyl-MARTKQTARKSTGGKAPRKQ
-
 Neurospora crassa