Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.35 extracted from

  • Urbonavicius, J.; Jaeger, G.; Bjoerk, G.R.
    Amino acid residues of the Escherichia coli tRNA(m5U54)methyltransferase (TrmA) critical for stability, covalent binding of tRNA and enzymatic activity (2007), Nucleic Acids Res., 35, 3297-3305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the plasmids pGP100, pDM4, pKD4 and pKD46 are used for the construction of the mutants Escherichia coli

Protein Variants

Protein Variants Comment Organism
C324A level of total TrmA-associated peptide 41%, amount of m5U in total tRNA 4%, relative to the levels found in the wild-type Escherichia coli
D299A level of total TrmA-associated peptide 63%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type Escherichia coli
E358K level of total TrmA-associated peptide 59%, amount of m5U in total tRNA 9%, relative to the levels found in the wild-type Escherichia coli
F188A level of total TrmA-associated peptide 84%, amount of m5U in total tRNA 22%, relative to the levels found in the wild-type Escherichia coli
G220D level of total TrmA-associated peptide 76%, amount of m5U in total tRNA 2%, relative to the levels found in the wild-type Escherichia coli
G360D level of total TrmA-associated peptide 53%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type Escherichia coli
Q190A level of total TrmA-associated peptide 77%, amount of m5U in total tRNA 14%, relative to the levels found in the wild-type Escherichia coli
R302A level of total TrmA-associated peptide 82%, amount of m5U in total tRNA 87%, relative to the levels found in the wild-type Escherichia coli
W132C level of total TrmA-associated peptide 102%, amount of m5U in total tRNA 34%, relative to the levels found in the wild-type Escherichia coli
W202C level of total TrmA-associated peptide 1%, amount of m5U in total tRNA 15%, relative to the levels found in the wild-type Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
native polypeptide, determined by SDS-PAGE and immunoblotting Escherichia coli
54000
-
complex containing a piece of the 3'-end of 16S rRNA Escherichia coli
62000
-
during the synthesis of m5U54 a covalent 62 kDa TrmA-tRNA intermediate is formed, determined by SDS-PAGE and immunoblotting Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + tRNA containing uridine at position 54 Escherichia coli
-
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + tRNA containing uridine at position 54
-
Escherichia coli S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
?

Synonyms

Synonyms Comment Organism
TrmA
-
Escherichia coli
tRNA(m5U54)methyltransferase
-
Escherichia coli
tRNA(uracil-5-)methyltransferase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Escherichia coli