Cloned (Comment) | Organism |
---|---|
the plasmids pGP100, pDM4, pKD4 and pKD46 are used for the construction of the mutants | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C324A | level of total TrmA-associated peptide 41%, amount of m5U in total tRNA 4%, relative to the levels found in the wild-type | Escherichia coli |
D299A | level of total TrmA-associated peptide 63%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type | Escherichia coli |
E358K | level of total TrmA-associated peptide 59%, amount of m5U in total tRNA 9%, relative to the levels found in the wild-type | Escherichia coli |
F188A | level of total TrmA-associated peptide 84%, amount of m5U in total tRNA 22%, relative to the levels found in the wild-type | Escherichia coli |
G220D | level of total TrmA-associated peptide 76%, amount of m5U in total tRNA 2%, relative to the levels found in the wild-type | Escherichia coli |
G360D | level of total TrmA-associated peptide 53%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type | Escherichia coli |
Q190A | level of total TrmA-associated peptide 77%, amount of m5U in total tRNA 14%, relative to the levels found in the wild-type | Escherichia coli |
R302A | level of total TrmA-associated peptide 82%, amount of m5U in total tRNA 87%, relative to the levels found in the wild-type | Escherichia coli |
W132C | level of total TrmA-associated peptide 102%, amount of m5U in total tRNA 34%, relative to the levels found in the wild-type | Escherichia coli |
W202C | level of total TrmA-associated peptide 1%, amount of m5U in total tRNA 15%, relative to the levels found in the wild-type | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
native polypeptide, determined by SDS-PAGE and immunoblotting | Escherichia coli |
54000 | - |
complex containing a piece of the 3'-end of 16S rRNA | Escherichia coli |
62000 | - |
during the synthesis of m5U54 a covalent 62 kDa TrmA-tRNA intermediate is formed, determined by SDS-PAGE and immunoblotting | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + tRNA containing uridine at position 54 | Escherichia coli | - |
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + tRNA containing uridine at position 54 | - |
Escherichia coli | S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrmA | - |
Escherichia coli |
tRNA(m5U54)methyltransferase | - |
Escherichia coli |
tRNA(uracil-5-)methyltransferase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |