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Literature summary for 2.1.1.35 extracted from

  • Nordlund, M.E.; Johansson, J.O.M.; von Pawel-Rammingen, U.; Byström, A.S.
    Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae (2000), RNA, 6, 844-860.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
cloning and sequencing of the TRM2 gene encoding enzyme, gene is previously identified as RNC1/NUD1 gene, but does not encode the yNucR endo-exonuclease, TRM2 gene is not essential for cell viability and contains alternative translational start sites, glutathione S-transferase tagged recombinant, Trm2p, is expressed in Escherichia coli Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus the m5U modification is found in unspliced tRNAs, suggesting that enzyme action is within the nucleus Saccharomyces cerevisiae 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uridine54 in tRNA Saccharomyces cerevisiae
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 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA enzyme is responsible for the formation of 5-methyluridine at position 54 in tRNA ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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 several strains
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Purification (Commentary)

Purification (Comment) Organism
purification of glutathione S-transferase tagged recombinant, Trm2p, expressed in Escherichia coli BL21 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uridine54 in tRNA
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 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA enzyme is responsible for the formation of 5-methyluridine at position 54 in tRNA ?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: yeast tRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA nearly all tRNAs contain m5U54, initiators excluded ?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: m5U-deficient tRNA from E. coli GB1-5-39 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA nearly all tRNAs contain m5U54, initiators excluded ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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 Saccharomyces cerevisiae