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Literature summary for 2.1.1.35 extracted from

  • Guenther, R.H.; Bakal, R.S.; Forrest, B.; Chen, Y.; Sengupta, R.; Nawrot, B.; Sochacka, E.; Jankowska, J.; Kraszewski, A.; Malkiewicz, A.; Agris, P.F.
    Aminoacyl-tRNA synthetase and U54 methyltransferase recognize conformations of the yeast tRNAPhe anticodon and T stem/loop domain (1994), Biochimie, 76, 1143-1151.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
DNA analog of the T-stem/loop domain DNA analog of the T-stem/loop domain of yeast tRNAPhe composed of d(A,U,G,C) inhibits the methylation of unfractionated methyl-deficient E. coli tRNA, significant inhibition when the concentration of tDNAPheT is twice that of the tRNA Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0057
-
uracil54 in unmodified T-stem/loop domain of yeast tRNA-Phe
-
Escherichia coli
0.0145
-
DNA analog of the T-stem/loop domain of yeast tRNAPhe
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli tRNA modifying enzyme S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli primary function of nucleoside modification within the tRNA anticodon loop is to negate potential canonical base pairs that would close the loop and, thereby, hinder codon binding S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA analog of the T-stem/loop domain of yeast tRNAPhe DNA analog of the T-stem/loop domain of yeast tRNAPhe composed of d(A,U,G,C) is also a substrate, slow methylation of tDNAPheT Escherichia coli S-adenosyl-L-homocysteine + DNA analog of the T-stem/loop domain of yeast tRNAPhe containing thymine
-
?
S-adenosyl-L-methionine + uracil54 in unmodified T-stem/loop domain of yeast tRNA-Phe
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified T-stem/loop domain of yeast tRNA-Phe
-
?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: unmodified yeast tRNAPhe Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptors: unmodified T-stem/loop domain of yeast tRNAPhe, methyl-deficient tRNA from E. coli C6 rel met- cys-, riboT-deficient tRNA from E. coli TrmA- is a specific substrate for U54 methylation, enzyme recognizes almost all tRNAs Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA tRNA modifying enzyme Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA primary function of nucleoside modification within the tRNA anticodon loop is to negate potential canonical base pairs that would close the loop and, thereby, hinder codon binding Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Escherichia coli