Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DNA analog of the T-stem/loop domain | DNA analog of the T-stem/loop domain of yeast tRNAPhe composed of d(A,U,G,C) inhibits the methylation of unfractionated methyl-deficient E. coli tRNA, significant inhibition when the concentration of tDNAPheT is twice that of the tRNA | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
uracil54 in unmodified T-stem/loop domain of yeast tRNA-Phe | - |
Escherichia coli | |
0.0145 | - |
DNA analog of the T-stem/loop domain of yeast tRNAPhe | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli | tRNA modifying enzyme | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli | primary function of nucleoside modification within the tRNA anticodon loop is to negate potential canonical base pairs that would close the loop and, thereby, hinder codon binding | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA analog of the T-stem/loop domain of yeast tRNAPhe | DNA analog of the T-stem/loop domain of yeast tRNAPhe composed of d(A,U,G,C) is also a substrate, slow methylation of tDNAPheT | Escherichia coli | S-adenosyl-L-homocysteine + DNA analog of the T-stem/loop domain of yeast tRNAPhe containing thymine | - |
? | |
S-adenosyl-L-methionine + uracil54 in unmodified T-stem/loop domain of yeast tRNA-Phe | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified T-stem/loop domain of yeast tRNA-Phe | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: unmodified yeast tRNAPhe | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptors: unmodified T-stem/loop domain of yeast tRNAPhe, methyl-deficient tRNA from E. coli C6 rel met- cys-, riboT-deficient tRNA from E. coli TrmA- is a specific substrate for U54 methylation, enzyme recognizes almost all tRNAs | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | tRNA modifying enzyme | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | primary function of nucleoside modification within the tRNA anticodon loop is to negate potential canonical base pairs that would close the loop and, thereby, hinder codon binding | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |