KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
uracil54 in yeast tRNA1-Phe | - |
Escherichia coli | |
0.004 | - |
uracil54 in 11mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | |
0.005 | - |
uracil54 in 17mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil54 in 11mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in 11mer of the T-arm of E. coli tRNA1-Val | - |
? | |
S-adenosyl-L-methionine + uracil54 in 17mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in 17mer of the T-arm of E. coli tRNA1-Val | - |
? | |
S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptors: E. coli tRNA1Val, 17-oligomer and 11-oligomer of the T-arm of tRNA1Val, essential sequence for tRNA binding: within the T-arm, composed of 7-base T-loop and 5-base pair stem, includes position 52 of T-stem, the T-loop and extends to the 3'-acceptor end, methylation site at U54 | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: unmodified yeast tRNAPhe | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptors: E. coli tRNAPhe, tRNAVal | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
uracil54 in 11mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | |
0.0333 | - |
uracil54 in 17mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | |
0.0917 | - |
uracil54 in yeast tRNA1-Phe | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |