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Literature summary for 2.1.1.35 extracted from
- Kinetic studies of Escherichia coli transfer RNA (uracil-5-)-methyltransferase (1978), Biochemistry, 17, 1068-1072.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| mature tRNA | weak, competitive inhibitor to methyl-deficient tRNA, non-competitive to S-adenosyl-L-methionine | Escherichia coli |  |
| S-adenosyl-L-homocysteine | non-competitive to tRNA; product inhibition, competitive to S-adenosyl-L-methionine | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
S-adenosyl-L-methionine | - |
Escherichia coli | |
| 0.025 | - |
methyl deficient tRNA | - |
Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight activation | Escherichia coli | |
| additional information | not activated by NH4+ | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli | specific reaction in maturation of tRNA | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli K12MO7 | specific reaction in maturation of tRNA | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli K12MO7 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA | kinetic, sequential mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000167 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -85°C, several months, stable | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Escherichia coli | additional information | - |
? | |
| additional information | - |
Escherichia coli K12MO7 | additional information | - |
? | |
| S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA | - |
Escherichia coli | ? | - |
? | |
| S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA | - |
Escherichia coli K12MO7 | ? | - |
? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: methyl-deficient tRNA from E. coli | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3 end | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | tRNA and S-adenosyl-L-methionine have separate and independent binding sites | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3 end | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | specific reaction in maturation of tRNA | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: methyl-deficient tRNA from E. coli | Escherichia coli K12MO7 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3 end | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | tRNA and S-adenosyl-L-methionine have separate and independent binding sites | Escherichia coli K12MO7 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3 end | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | specific reaction in maturation of tRNA | Escherichia coli K12MO7 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | 0.004 | S-adenosyl-L-homocysteine | - |
Escherichia coli | |
| 0.03 | 0.1 | mature tRNA | - |
Escherichia coli | |
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