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Literature summary for 2.1.1.35 extracted from
- Bacterial tRNA methyltransferases (1979), Methods Enzymol., 59, 190-203.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | enzyme is used for detecting tRNA-like moieties in viral RNA | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-homocysteine | at 0.002 mM 50% inhibition, E. coli MRE 600 | Escherichia coli |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Escherichia coli | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Enterococcus faecalis | - |
- |
- |
| Escherichia coli | - |
MRE 600 | - |
| Escherichia coli MRE 600 | - |
MRE 600 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity elution and hydroxylapatite chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00134 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, at least 6 months, stable | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Escherichia coli | additional information | - |
? | |
| additional information | tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine | Bacillus subtilis | additional information | - |
? | |
| additional information | tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine | Enterococcus faecalis | additional information | - |
? | |
| additional information | tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine | Bacillus cereus | additional information | - |
? | |
| additional information | - |
Escherichia coli MRE 600 | additional information | - |
? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | high substrate specificity, methyl group acceptors: wheat germ tRNA1Gly, tRNA2Gly, E. coli tRNA1Ala | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | high substrate specificity, methyl group acceptors: wheat germ tRNA1Gly, tRNA2Gly, E. coli tRNA1Ala | Escherichia coli MRE 600 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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