General Stability | Organism |
---|---|
freezing without antifreeze agents inactivates, 30% ethylene glycol at -20°C stabilizes, dialysis inactivates, best pH used for dialysis is 6.5 | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | inhibits at low concentrations | Saccharomyces cerevisiae | |
Mn2+ | inhibits at low concentrations | Saccharomyces cerevisiae | |
NH4+ | at 0.1 M, 50% inhibition | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no divalent cations required, not activated by Mg2+ and Mn2+ | Saccharomyces cerevisiae |
Organic Solvent | Comment | Organism |
---|---|---|
Ethylene glycol | 30%, stabilizes at -20°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae D84 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
may be 3 isoenzymes | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: methyl-deficient tRNA from E. coli | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: methyl-deficient tRNA from E. coli | Saccharomyces cerevisiae D84 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae |