Literature summary for 2.1.1.348 extracted from

Li, F.; Kennedy, S.; Hajian, T.; Gibson, E.; Seitova, A.; Xu, C.; Arrowsmith, C.H.; Vedadi, M.
A radioactivity-based assay for screening human m6A-RNA methyltransferase, METTL3-METTL14 complex, and demethylase ALKBH5 (2016), J. Biomol. Screen., 21, 290-297 .

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Application

Application	Comment	Organism
analysis	radioactivity-based assays using 3H-S-adenosyl-L-methionine for kinetic characterization of m6A-RNA modifications by METTL3-14 complex and m6A-RNA demethylase ALKBH5. Assay conditions are suitable for screening for ligands in a 384-well format with Z' factors of 0.78 and 0.77, respectively	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Expi293 cells	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q86U44 and Q9HCE5	Q86U44 i.e. catalytic subunit METTL3, Q9HCE5 i.e non-catalytic subunit METTL14	-

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Release 2023.1 (January 2023)