Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.343 extracted from

  • Jankowitsch, F.; Kühm, C.; Kellner, R.; Kalinowski, J.; Pelzer, S.; Macheroux, P.; Mack, M.
    A novel N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis (2011), J. Biol. Chem., 286, 38275-38285 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells Streptomyces davaonensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0577
-
8-amino-8-demethyl-D-riboflavin apparent value, at pH 8.0 and 37°C Streptomyces davaonensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin Streptomyces davaonensis
-
S-adenosyl-L-homocysteine + roseoflavin
-
?
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin Streptomyces davaonensis 768
-
S-adenosyl-L-homocysteine + roseoflavin
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces davaonensis E7BBN5
-
-
Streptomyces davaonensis 768 E7BBN5
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Streptomyces davaonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
-
Streptomyces davaonensis S-adenosyl-L-homocysteine + roseoflavin
-
?
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
-
Streptomyces davaonensis 768 S-adenosyl-L-homocysteine + roseoflavin
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Streptomyces davaonensis

Synonyms

Synonyms Comment Organism
N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase
-
Streptomyces davaonensis
RosA
-
Streptomyces davaonensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
8-amino-8-demethyl-D-riboflavin at pH 8.0 and 37°C Streptomyces davaonensis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Streptomyces davaonensis