Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) and BL834 (DE3) cells | Streptomyces davaonensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
roseoflavin | competitive inhibition | Streptomyces davaonensis | |
S-adenosyl-L-homocysteine | competitive inhibition | Streptomyces davaonensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
8-amino-8-demethyl-D-riboflavin | at pH 8.0 and 25°C | Streptomyces davaonensis | |
0.07 | - |
S-adenosyl-L-methionine | at pH 8.0 and 25°C | Streptomyces davaonensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
gel filtration | Streptomyces davaonensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin | Streptomyces davaonensis | - |
S-adenosyl-L-homocysteine + roseoflavin | - |
? | |
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin | Streptomyces davaonensis JCM 4913 | - |
S-adenosyl-L-homocysteine + roseoflavin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces davaonensis | K4RFM2 | - |
- |
Streptomyces davaonensis JCM 4913 | K4RFM2 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and MonoQ column chromatography | Streptomyces davaonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin | - |
Streptomyces davaonensis | S-adenosyl-L-homocysteine + roseoflavin | - |
? | |
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin | - |
Streptomyces davaonensis JCM 4913 | S-adenosyl-L-homocysteine + roseoflavin | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, SDS-PAGE | Streptomyces davaonensis |
Synonyms | Comment | Organism |
---|---|---|
RosA | - |
Streptomyces davaonensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
S-adenosyl-L-methionine | at pH 8.0 and 25°C | Streptomyces davaonensis | |
0.001 | - |
8-amino-8-demethyl-D-riboflavin | at pH 8.0 and 25°C | Streptomyces davaonensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Streptomyces davaonensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
roseoflavin | at pH 8.0 and 25°C | Streptomyces davaonensis | |
0.027 | - |
S-adenosyl-L-homocysteine | at pH 8.0 and 25°C | Streptomyces davaonensis |