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Literature summary for 2.1.1.343 extracted from

  • Tongsook, C.; Uhl, M.; Jankowitsch, F.; Mack, M.; Gruber, K.; Macheroux, P.
    Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-D-riboflavin to the antibiotic roseoflavin (2016), FEBS J., 283, 1531-1549 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.343

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) and BL834 (DE3) cells Streptomyces davaonensis

Inhibitors

Inhibitors Comment Organism Structure
roseoflavin competitive inhibition Streptomyces davaonensis
S-adenosyl-L-homocysteine competitive inhibition Streptomyces davaonensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 8-amino-8-demethyl-D-riboflavin at pH 8.0 and 25°C Streptomyces davaonensis
0.07
-
 S-adenosyl-L-methionine at pH 8.0 and 25°C Streptomyces davaonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 gel filtration Streptomyces davaonensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin Streptomyces davaonensis
-
 S-adenosyl-L-homocysteine + roseoflavin
-
 ?
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin Streptomyces davaonensis JCM 4913
-
 S-adenosyl-L-homocysteine + roseoflavin
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces davaonensis K4RFM2
-
-
Streptomyces davaonensis JCM 4913 K4RFM2
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and MonoQ column chromatography Streptomyces davaonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
-
 Streptomyces davaonensis S-adenosyl-L-homocysteine + roseoflavin
-
 ?
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
-
 Streptomyces davaonensis JCM 4913 S-adenosyl-L-homocysteine + roseoflavin
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, SDS-PAGE Streptomyces davaonensis

Synonyms

Synonyms Comment Organism
RosA
-
 Streptomyces davaonensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 S-adenosyl-L-methionine at pH 8.0 and 25°C Streptomyces davaonensis
0.001
-
 8-amino-8-demethyl-D-riboflavin at pH 8.0 and 25°C Streptomyces davaonensis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Streptomyces davaonensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
 roseoflavin at pH 8.0 and 25°C Streptomyces davaonensis
0.027
-
 S-adenosyl-L-homocysteine at pH 8.0 and 25°C Streptomyces davaonensis