Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3), recombinant His6-tagged RosA purified, ORF sda77220 is named rosA is located in the middle of a putative gene cluster within the genome of Streptomyces davawensis | Streptomyces davaonensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0286 | - |
S-adenosyl-L-methionine | pH 8.0, 37°C | Streptomyces davaonensis | |
0.0577 | - |
8-amino-8-demethyl-D-riboflavin | pH 8.0, 37°C | Streptomyces davaonensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | cell-free extract, roseoflavin synthesis is observed in the exponentially phase growth, under laboratory conditions using starch and yeast extract containing broth, roseoflavin synthesis is observed in the stationary phase of growth | Streptomyces davaonensis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37940 | - |
calculated | Streptomyces davaonensis |
38000 | - |
1 * 38000, calculated, purified recombinant RosA | Streptomyces davaonensis |
40000 | - |
SDS-PAGE | Streptomyces davaonensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine | Streptomyces davaonensis | - |
roseoflavin + S-adenosyl-L-homocysteine | the products isolated by HPLC and identified by mass spectrometry | ir | |
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine | Streptomyces davaonensis 768 | - |
roseoflavin + S-adenosyl-L-homocysteine | the products isolated by HPLC and identified by mass spectrometry | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces davaonensis | E7BBN5 | - |
- |
Streptomyces davaonensis 768 | E7BBN5 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Sepharose chromatography, anion exchange chromatography, gel filtration on a HiLoad 16/60 Superdex 200 column, analyzed using SDS-PAGE and MS/MS | Streptomyces davaonensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin | roseoflavin, i.e. 1-deoxy-1-[8-(dimethylamino)-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl]pentitol, a two-step methylation reaction | Streptomyces davaonensis | |
S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin | 1a | Streptomyces davaonensis | |
S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin | 1b | Streptomyces davaonensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.58 | - |
pH 8.0, 37°C | Streptomyces davaonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine | - |
Streptomyces davaonensis | roseoflavin + S-adenosyl-L-homocysteine | the products isolated by HPLC and identified by mass spectrometry | ir | |
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine | - |
Streptomyces davaonensis | roseoflavin + S-adenosyl-L-homocysteine | products isolated by HPLC and identified by mass spectrometry | ir | |
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine | - |
Streptomyces davaonensis 768 | roseoflavin + S-adenosyl-L-homocysteine | the products isolated by HPLC and identified by mass spectrometry | ir | |
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine | - |
Streptomyces davaonensis 768 | roseoflavin + S-adenosyl-L-homocysteine | products isolated by HPLC and identified by mass spectrometry | ir | |
additional information | no activity with 8-hydroxy-8-demethyl-D-riboflavin | Streptomyces davaonensis | ? | - |
? | |
additional information | no activity with 8-hydroxy-8-demethyl-D-riboflavin | Streptomyces davaonensis 768 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, calculated, purified recombinant RosA | Streptomyces davaonensis |
Synonyms | Comment | Organism |
---|---|---|
N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase | - |
Streptomyces davaonensis |
RosA | rosA: gene name | Streptomyces davaonensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces davaonensis |
52 | - |
- |
Streptomyces davaonensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
S-adenosyl-L-methionine | pH 8.0, 37°C | Streptomyces davaonensis | |
0.37 | - |
8-amino-8-demethyl-D-riboflavin | pH 8.0, 37°C | Streptomyces davaonensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces davaonensis |
8.8 | - |
- |
Streptomyces davaonensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Streptomyces davaonensis |
General Information | Comment | Organism |
---|---|---|
physiological function | RosA catalyzes the two terminal steps in the biosynthesis of the antibiotic roseoflavin. RosA is the first enzyme of the biosynthesis to be identified | Streptomyces davaonensis |