Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.34 extracted from

  • Watanabe, K.; Nureki, O.; Fukai, S.; Ishii, R.; Okamoto, H.; Yokoyama, S.; Endo, Y.; Hori, H.
    Roles of conserved amino acid sequence motifs in the SpoU (TrmH) RNA methyltransferase family (2005), J. Biol. Chem., 280, 10368-10377.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E124A extensive structural alterations compared to wild-type, marked decrease in activity Thermus thermophilus
E124D small increase in Km-value Thermus thermophilus
E124Q no enzymic activity Thermus thermophilus
N152A extensive structural alterations compared to wild-type, marked decrease in activity Thermus thermophilus
N152D no enzymic activity Thermus thermophilus
N152E no enzymic activity Thermus thermophilus
N35A marked decrease in methyl transfer activity Thermus thermophilus
N35D marked decrease in Km-value for tRNA, low affinity for S-adenosyl-L-homocysteine Thermus thermophilus
N35Q dramatic increase in Km-value Thermus thermophilus
P143Q small increase in Km-value Thermus thermophilus
R41M large increase in Km-value Thermus thermophilus
S150C Km-value similar to wild-type, decrease in Vmax Thermus thermophilus
S150T 4fold increase in Km-value Thermus thermophilus
S37G 5fold increase in Km-value Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
S-adenosyl-L-methionine pH 7.5, 50°C, wild-type Thermus thermophilus
0.011
-
S-adenosyl-L-methionine pH 7.5, 50°C, mutant S150C Thermus thermophilus
0.014
-
S-adenosyl-L-methionine pH 7.5, 50°C, mutant E124D Thermus thermophilus
0.014
-
S-adenosyl-L-methionine pH 7.5, 50°C, mutant P143Q Thermus thermophilus
0.037
-
S-adenosyl-L-methionine pH 7.5, 50°C, mutant S150T Thermus thermophilus
0.051
-
S-adenosyl-L-methionine pH 7.5, 50°C, mutant S37G Thermus thermophilus
0.083
-
S-adenosyl-L-methionine pH 7.5, 50°C, mutant N35A Thermus thermophilus
0.67
-
S-adenosyl-L-methionine pH 7.5, 50°C, mutant N35D Thermus thermophilus
0.92
-
S-adenosyl-L-methionine pH 7.5, 50°C, mutant R41M Thermus thermophilus
2.1
-
S-adenosyl-L-methionine pH 7.5, 50°C, mutant N35Q Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme hydroxylates guanosine at postion 18 in tRNA Thermus thermophilus ?
-
?
S-adenosyl-L-methionine + yeast tRNA-Phe
-
Thermus thermophilus S-adenosyl-L-homocysteine + yeast tRNA-Phe containing 2'-O-methylguanine
-
?

Synonyms

Synonyms Comment Organism
SpoU
-
Thermus thermophilus
TrmH
-
Thermus thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Thermus thermophilus
-
-
9.63