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Literature summary for 2.1.1.34 extracted from

  • Kumagai, I.; Watanabe, K.; Oshima, T.
    A thermostable tRNA (guanosine-2)-methyltransferase from Thermus thermophilus HB27 and the effect of ribose methylation on the conformational stability of tRNA (1982), J. Biol. Chem., 257, 7388-7395.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
spermine activation, 5 mM Thermus thermophilus

General Stability

General Stability Organism
urea, stable to 6 M Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine strong, product inhibition, competitive Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00001
-
guanosine18 in Escherichia coli tRNA-Phe
-
Thermus thermophilus
0.00047
-
S-adenosyl-L-methionine
-
Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation, 5 mM Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
gel filtration Thermus thermophilus
21000
-
1 * 21000, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanosine18 in tRNATyr Thermus thermophilus involved in post-transcriptional tRNA modification S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
?
S-adenosyl-L-methionine + guanosine18 in tRNATyr Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 involved in post-transcriptional tRNA modification S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus
-
high growth temperature enhances activity
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on S-adenosyl-L-homocysteine-Sepharose, elution with 6 M urea Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0071
-
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus ?
-
?
additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
?
S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Phe
-
Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Phe
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNATyr involved in post-transcriptional tRNA modification Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
?
S-adenosyl-L-methionine + guanosine18 in tRNATyr involved in post-transcriptional tRNA modification Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
?

Subunits

Subunits Comment Organism
monomer 1 * 21000, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
about 90% of original activity retained after 20 min in the presence of 10% v/v glycerol at pH 7.5 Thermus thermophilus
90
-
t1/2: 20 min Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.5
-
Thermus thermophilus