Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermine | activation, 5 mM | Thermus thermophilus |
General Stability | Organism |
---|---|
urea, stable to 6 M | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | strong, product inhibition, competitive | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
guanosine18 in Escherichia coli tRNA-Phe | - |
Thermus thermophilus | |
0.00047 | - |
S-adenosyl-L-methionine | - |
Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation, 5 mM | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
gel filtration | Thermus thermophilus |
21000 | - |
1 * 21000, SDS-PAGE | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanosine18 in tRNATyr | Thermus thermophilus | involved in post-transcriptional tRNA modification | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNATyr | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | involved in post-transcriptional tRNA modification | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus | - |
high growth temperature enhances activity | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography on S-adenosyl-L-homocysteine-Sepharose, elution with 6 M urea | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0071 | - |
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli | Thermus thermophilus | ? | - |
? | |
additional information | specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? | |
S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Phe | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Phe | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptor from yeast: tRNAPhe | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptor from yeast: tRNAPhe | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNATyr | involved in post-transcriptional tRNA modification | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNATyr | involved in post-transcriptional tRNA modification | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 21000, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
about 90% of original activity retained after 20 min in the presence of 10% v/v glycerol at pH 7.5 | Thermus thermophilus |
90 | - |
t1/2: 20 min | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.5 | - |
Thermus thermophilus |