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Literature summary for 2.1.1.33 extracted from

  • Zegers, I.; Gigot, D.; van Vliet, F.; Tricot, C.; Aymerich, S.; Bujnicki, J.M.; Kosinski, J.; Droogmans, L.
    Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase (2006), Nucleic Acids Res., 34, 1925-1934.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
by hanging drop method, at a resolution of 2.1 A, Rossmann-fold methyltransferase structure with the N-terminal helix folded on the opposite site of the catalytic domain Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information mutant strain, trmB gene inactivated by an insertion, shows normal growth Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ contains three potassium ions per asymmetric unit, one in the interface between monomers and one in a region involved in AdoMet binding in the homologous catechol O-MTase, bound to the main chain carbonyls of residues Asn115 and Gly46 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34522
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Subunits

Subunits Comment Organism
dimer crystallographic studies, gel filtration, dimeric both in crystal and in solution Bacillus subtilis

Synonyms

Synonyms Comment Organism
TrmB
-
Bacillus subtilis
tRNA (m7G46) methyltransferase
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Bacillus subtilis