Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, genetic organization and clustering of SAM-dependent methyltransferase sequences, phylogenetic tree, recombinant enzyme functional expression in a Saccharomyces cerevisiae strain that is engineered to produce glucosylceramides suitable as a substrate for C9-methylation. C9-methylated sphingolipids are detected in the transformed strain expressing the candidate from Pichia pastoris, demonstrating its function as a sphingolipid C9-methyltransferase | Komagataella pastoris |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a Pichia pastoris knock-out strain lacking sphingolipid C9-methyltransferase, the strain contains only non-methylated glucosylceramides | Komagataella pastoris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Komagataella pastoris | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + a (4E,8E)-sphinga-4,8-dienine ceramide | Komagataella pastoris | - |
S-adenosyl-L-homocysteine + a 9-methyl-(4E,8E)-sphinga-4,8-dienine ceramide | - |
? | |
S-adenosyl-L-methionine + a (4E,8E)-sphinga-4,8-dienine ceramide | Komagataella pastoris GS115 | - |
S-adenosyl-L-homocysteine + a 9-methyl-(4E,8E)-sphinga-4,8-dienine ceramide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | Q2QJ12 | i.e. Pichia pastoris | - |
Komagataella pastoris GS115 | Q2QJ12 | i.e. Pichia pastoris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + a (4E,8E)-sphinga-4,8-dienine ceramide | - |
Komagataella pastoris | S-adenosyl-L-homocysteine + a 9-methyl-(4E,8E)-sphinga-4,8-dienine ceramide | - |
? | |
S-adenosyl-L-methionine + a (4E,8E)-sphinga-4,8-dienine ceramide | - |
Komagataella pastoris GS115 | S-adenosyl-L-homocysteine + a 9-methyl-(4E,8E)-sphinga-4,8-dienine ceramide | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Komagataella pastoris |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the superfamily of S-adenosylmethionine-dependent methyltransferases and shows highest sequence similarity to plant and bacterial cyclopropane fatty acid synthases, genetic organization and clustering of SAM-dependent methyltransferase sequences, phylogenetic profiling of SAM-dependent methyltransferases, overview | Komagataella pastoris |
malfunction | a Pichia pastoris knock-out strain of the sphingolipid C9-methyltransferase lacks C9-methylated glucosylceramides | Komagataella pastoris |
additional information | analysis of the sphingoid base composition of wild-type strains and Saccharomyces cerevisiae strain recombinantly expressing the enzyme | Komagataella pastoris |