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Literature summary for 2.1.1.316 extracted from

  • Singh, S.; Chang, A.; Goff, R.D.; Bingman, C.A.; Grschow, S.; Sherman, D.H.; Phillips, G.N. Jr.; Thorson, J.S.
    Structural characterization of the mitomycin 7-O-methyltransferase (2011), Proteins, 79, 2181-2188.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.316

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as N-terminal polyhistidine-tagged protein Streptomyces lavendulae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, crystal structures of the mitomycin-7-O-methyltransferase–S-adenosylhomocysteine binary complex and mitomycin-7-O-methyltransferase–S-adenosyl-L-homocysteine–mitomycin A ternary complex at resolutions of 1.9 and 2.3 A, respectively Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptomyces lavendulae the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin ?
-
 ?
additional information Streptomyces lavendulae NRRL 2564 the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae Q9X5T6
-
-
Streptomyces lavendulae NRRL 2564 Q9X5T6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces lavendulae

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 6-demethylmitomycin A = S-adenosyl-L-homocysteine + mitomycin A (1) Streptomyces lavendulae
a
S-adenosyl-L-methionine + 6-demethylmitomycin B = S-adenosyl-L-homocysteine + mitomycin B (2) Streptomyces lavendulae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin Streptomyces lavendulae ?
-
 ?
additional information the enzyme catalyzes the 7-O-methylation of both C9beta- and C9alpha-configured 7-hydroxymitomycins Streptomyces lavendulae ?
-
 ?
additional information the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin Streptomyces lavendulae NRRL 2564 ?
-
 ?
additional information the enzyme catalyzes the 7-O-methylation of both C9beta- and C9alpha-configured 7-hydroxymitomycins Streptomyces lavendulae NRRL 2564 ?
-
 ?
S-adenosyl-L-methionine + 6-demethylmitomycin A
-
 Streptomyces lavendulae S-adenosyl-L-homocysteine + mitomycin A
-
 ?
S-adenosyl-L-methionine + 6-demethylmitomycin A
-
 Streptomyces lavendulae NRRL 2564 S-adenosyl-L-homocysteine + mitomycin A
-
 ?
S-adenosyl-L-methionine + 6-demethylmitomycin B
-
 Streptomyces lavendulae S-adenosyl-L-homocysteine + mitomycin B
-
 ?
S-adenosyl-L-methionine + 6-demethylmitomycin B
-
 Streptomyces lavendulae NRRL 2564 S-adenosyl-L-homocysteine + mitomycin B
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Streptomyces lavendulae

Synonyms

Synonyms Comment Organism
MmcR
-
 Streptomyces lavendulae

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin Streptomyces lavendulae