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Literature summary for 2.1.1.314 extracted from

  • Mayer, K.; Schröder, A.; Schnitger, J.; Stahl, S.; Brinkmann, U.
    Influence of DPH1 and DPH5 protein variants on the synthesis of diphthamide, the target of ADPRibosylating toxins (2017), Toxins, 9, E78 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.314

Cloned(Commentary)

Cloned (Comment) Organism
expression of DPH1 and DPH5 cDNA in DPH deficient MCF7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D57G mutant retains activity Homo sapiens
delA212 frequently occurring DPH5 isoform retained activity Homo sapiens
E60stop inactive mutant containing intact N-terminus with truncations after amino acid 207 Homo sapiens
G87R mutant retains activity Homo sapiens
Q170H mutant retains activity Homo sapiens
R207stop inactive mutant containing intact N-terminus with truncations after amino acid 207 Homo sapiens
S123C mutant retains activity Homo sapiens
W136stop inactive mutant containing intact N-terminus with truncations after amino acid 207 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] Homo sapiens
-
 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H2P9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2]
-
 Homo sapiens 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
-
 ?

Synonyms

Synonyms Comment Organism
Dph5
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function DPH1 and DPH5 are essential to generate diphthamide on eukaryotic translation elongation factor 2, the sole target of ADP-ribosylating toxins. DPH1 and DPH5-deficient MCF7 are therefore resistant to ADP-ribosylation and cytotoxicity inflicted by toxins that target eEF2-diphthamide Homo sapiens