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Literature summary for 2.1.1.313 extracted from

  • Sharma, S.; Yang, J.; Duettmann, S.; Watzinger, P.; Koetter, P.; Entian, K.D.
    Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae (2014), Nucleic Acids Res., 42, 3246-3260.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.313

Cloned(Commentary)

Cloned (Comment) Organism
plasmid pSH26 carrying N terminal 6xHis-Smt3–tagged Bmt5, is constructed using pPK591 by gap repair. For overexpression, BL21 (DE3) cells are transformed with isolated plasmids and grown in LB medium containing 100 mg/ml ampicillin at 37°C to an OD600 of 0.6 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleolus
-
 Saccharomyces cerevisiae 5730
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uracil2634 in 25S rRNA Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
-
 ?
S-adenosyl-L-methionine + uracil2634 in 25S rRNA Saccharomyces cerevisiae ATCC 204508
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 S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P40493
-
-
Saccharomyces cerevisiae ATCC 204508 P40493
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged enzyme is purified by Nickel-NTA metal affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uracil2634 in 25S rRNA
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
-
 ?
S-adenosyl-L-methionine + uracil2634 in 25S rRNA
-
 Saccharomyces cerevisiae ATCC 204508 S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
BMT5
-
 Saccharomyces cerevisiae
YIL096C locus name Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction loss of bmt5 does not impair the growth of the cells at 30°C, 37°C and 19°C on YPD media. The loss of bmt5 does not exhibit any hypersensitivity to paromomycin and anisomycin. The absence of the N3-methyluracil2634 modification does not disturb ribosomal stoichiometry and affect translation Saccharomyces cerevisiae