Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | activates | Streptomyces carzinostaticus |
Cloned (Comment) | Organism |
---|---|
NcsB1 is overproduced as an N-terminal His6-tagged fusion protein in Escherichia coli BL21(DE3) | Streptomyces carzinostaticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete loss of activity | Streptomyces carzinostaticus | |
Mg2+ | - |
Streptomyces carzinostaticus | |
Zn2+ | - |
Streptomyces carzinostaticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
1,4-dihydroxy-2-naphthoic acid | pH 6.0, 25°C | Streptomyces carzinostaticus | |
0.066 | - |
3,7-dihydroxy-2-naphthoate | pH 6.0, 25°C | Streptomyces carzinostaticus | |
0.206 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 6.0, 25°C | Streptomyces carzinostaticus | |
0.352 | - |
3,5-dihydroxy-2-naphthoate | pH 6.0, 25°C | Streptomyces carzinostaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | increases activity | Streptomyces carzinostaticus | |
Mn2+ | increases activity | Streptomyces carzinostaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39500 | - |
x * 39500, SDS-PAGE | Streptomyces carzinostaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 2,7-dihydroxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | S-adenosyl-L-homocysteine + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces carzinostaticus | Q84HC8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces carzinostaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NcsB1 also recognizes other dihydroxynaphthoic acids as substrates and catalyzes regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids | Streptomyces carzinostaticus | ? | - |
? | |
S-adenosyl-L-methionine + 1,4-dihydroxy-2-naphthoic acid | kcat/KM is 10% compared to the wild-type value | Streptomyces carzinostaticus | S-adenosyl-L-homocysteine + 1-hydroxy-4-methoxy-2-naphthoate | - |
? | |
S-adenosyl-L-methionine + 2,7-dihydroxy-5-methyl-1-naphthoate | - |
Streptomyces carzinostaticus | S-adenosyl-L-homocysteine + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | - |
? | |
S-adenosyl-L-methionine + 2,7-dihydroxy-5-methyl-1-naphthoate | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus | S-adenosyl-L-homocysteine + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | - |
? | |
S-adenosyl-L-methionine + 3,5-dihydroxy-2-naphthoate | kcat/KM is 23% compared to the wild-type value | Streptomyces carzinostaticus | S-adenosyl-L-homocysteine + 3-hydroxy-5-methoxy-2-naphthoate | - |
? | |
S-adenosyl-L-methionine + 3,7-dihydroxy-2-naphthoate | kcat/KM is 14% compared to the wild-type value | Streptomyces carzinostaticus | S-adenosyl-L-homocysteine + 3-hydroxy-7-methoxy-2-naphthoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39500, SDS-PAGE | Streptomyces carzinostaticus |
Synonyms | Comment | Organism |
---|---|---|
NcsB1 | - |
Streptomyces carzinostaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces carzinostaticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000133 | - |
1,4-dihydroxy-2-naphthoic acid | pH 6.0, 25°C | Streptomyces carzinostaticus | |
0.0004 | - |
3,7-dihydroxy-2-naphthoate | pH 6.0, 25°C | Streptomyces carzinostaticus | |
0.0045 | - |
3,5-dihydroxy-2-naphthoate | pH 6.0, 25°C | Streptomyces carzinostaticus | |
0.0115 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 6.0, 25°C | Streptomyces carzinostaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptomyces carzinostaticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: optimum, pH 8.0: about 50% of maximal activity, no activity below pH 5.5 | Streptomyces carzinostaticus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
1,4-dihydroxy-2-naphthoic acid | pH 6.0, 25°C | Streptomyces carzinostaticus | |
0.0061 | - |
3,7-dihydroxy-2-naphthoate | pH 6.0, 25°C | Streptomyces carzinostaticus | |
0.013 | - |
3,5-dihydroxy-2-naphthoate | pH 6.0, 25°C | Streptomyces carzinostaticus | |
0.06 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 6.0, 25°C | Streptomyces carzinostaticus |