Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyclic-di-GMP | WarA binds to cyclic-di-GMP, which potentiates its methyltransferase activity | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
gene warA, quantitative RT-PCR expression analysis, recombinant expression of N-terminally His- or GFP-tagged enzyme, recombinat expression of GST-His-tagged wild-type and mutant enzymes in Escherichia coli strain B834(DE3) | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a gene replacement mutants, deletion of the PA4379 (warA) gene in the Pseudomonas aeruginosa PAK chromosome | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | ? | - |
- |
|
additional information | Pseudomonas aeruginosa ATCC 15692 | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | ? | - |
- |
|
additional information | Pseudomonas aeruginosa 1C | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | ? | - |
- |
|
additional information | Pseudomonas aeruginosa PRS 101 | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | ? | - |
- |
|
additional information | Pseudomonas aeruginosa DSM 22644 | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | ? | - |
- |
|
additional information | Pseudomonas aeruginosa CIP 104116 | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | ? | - |
- |
|
additional information | Pseudomonas aeruginosa LMG 12228 | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | ? | - |
- |
|
additional information | Pseudomonas aeruginosa JCM 14847 | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HW23 | - |
- |
Pseudomonas aeruginosa 1C | Q9HW23 | - |
- |
Pseudomonas aeruginosa ATCC 15692 | Q9HW23 | - |
- |
Pseudomonas aeruginosa CIP 104116 | Q9HW23 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9HW23 | - |
- |
Pseudomonas aeruginosa JCM 14847 | Q9HW23 | - |
- |
Pseudomonas aeruginosa LMG 12228 | Q9HW23 | - |
- |
Pseudomonas aeruginosa PRS 101 | Q9HW23 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinat GST-His-tagged wild-type and mutant enzymes from Escherichia coli strain B834(DE3) by nickel affinity chromatography and gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | Pseudomonas aeruginosa | ? | - |
- |
|
additional information | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | Pseudomonas aeruginosa ATCC 15692 | ? | - |
- |
|
additional information | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | Pseudomonas aeruginosa 1C | ? | - |
- |
|
additional information | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | Pseudomonas aeruginosa PRS 101 | ? | - |
- |
|
additional information | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | Pseudomonas aeruginosa DSM 22644 | ? | - |
- |
|
additional information | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | Pseudomonas aeruginosa CIP 104116 | ? | - |
- |
|
additional information | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | Pseudomonas aeruginosa LMG 12228 | ? | - |
- |
|
additional information | SadC interacts with the methyltransferase WarA, identification of the protein-protein interaction network surrounding the central switch component SadC, overview. Bacterial two-hybrid assay | Pseudomonas aeruginosa JCM 14847 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
methyltransf_25 domain-containing protein | UniProt | Pseudomonas aeruginosa |
WarA | - |
Pseudomonas aeruginosa |
WbdD-like O antigen regulator | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
evolution | WarA and WarB have structural similarities with the bi-functional Escherichia coli LPS O antigen regulator WbdD. A predicted three-dimensional model is generated with a very high confidence on the Escherichia coli serotype O9a WbdD protein (19% sequence identity, 69% sequence coverage, 100% confidence) | Pseudomonas aeruginosa |
malfunction | The immune response to Pseudomonas aeruginosa in a Pseudomonas-zebrafish hindbrain infection model shows that deletion of warA or sadC does not reduce bacterial load. A phenotype of significantly increased neutrophil recruitment is observed for a warA deletion mutant. Infection with a warA mutant also induces TNF-alpha mRNA levels significantly higher than wild-type infected larvae | Pseudomonas aeruginosa |
additional information | WarA is a methyltransferase in complex with a putative kinase WarB. WarA binds to cyclic-di-GMP, which potentiates its methyltransferase activity. Bacterial two-hybrid assay | Pseudomonas aeruginosa |
physiological function | WarA influences Pseudomonas aeruginosa O antigen modal distribution and interacts with the LPS biogenesis machinery. LPS is known to modulate the immune response in the host, WarA is involved in the ability of Pseudomonas aeruginosa to evade detection by the host. Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion. WarA might play a similar functional role as WbdD in regulating chain length and termination of LPS O antigen synthesis. The immune response to Pseudomonas aeruginosa in a Pseudomonas-zebrafish hindbrain infection model shows that deletion of warA or sadC does not reduce bacterial load. The regulation of LPS by SadC and WarA plays a critical role in immune cell evasion during infection | Pseudomonas aeruginosa |