Any feedback?
Literature summary for 2.1.1.287 extracted from
- A single N1-methyladenosine on the large ribosomal subunit rRNA impacts locally its structure and the translation of key metabolic enzymes (2018), Sci. Rep., 8, 11904 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rrp8, sequence comparisons and phylogenetic analysis, quantitative enzyme expression analysis | Saccharomyces cerevisiae |
| gene rrp8, sequence comparisons and phylogenetic analysis, quantitative enzyme expression analysis, recombinant expression in and complementation of Saccharomyces cereviaie Rrp8 deficient mutant strain | Candida albicans |
| gene rrp8, sequence comparisons and phylogenetic analysis, quantitative enzyme expression analysis, recombinant expression in Saccharomyces cereviaie Rrp8 deficient mutant strain without complementation by the Schizosaccharomyces pombe Rrp8 enzyme | Schizosaccharomyces pombe |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G290R | rrp8G209R loss of methylation mutant, catalytically dead mutant, the loss of m1A645 leads to structural changes in and around the helix 25.1 region of the 25S rRNA, phenotypes, detailed overview | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Candida albicans | 5634 | - |
| nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Candida albicans | 25S/28S rRNA of Candida albicans contains a single m1A methylation in the helix 25.1 | ? | - |
- |
 |
| additional information | Schizosaccharomyces pombe | 25S/28S rRNA of Schizosaccharomyces pombe contains a single m1A methylation in the helix 25.1 | ? | - |
- |
|
| additional information | Candida albicans CBS 356 | 25S/28S rRNA of Candida albicans contains a single m1A methylation in the helix 25.1 | ? | - |
- |
|
| additional information | Schizosaccharomyces pombe ATCC 24843 | 25S/28S rRNA of Schizosaccharomyces pombe contains a single m1A methylation in the helix 25.1 | ? | - |
- |
|
| additional information | Schizosaccharomyces pombe 972 | 25S/28S rRNA of Schizosaccharomyces pombe contains a single m1A methylation in the helix 25.1 | ? | - |
- |
|
| additional information | Candida albicans RM1000 | 25S/28S rRNA of Candida albicans contains a single m1A methylation in the helix 25.1 | ? | - |
- |
|
| S-adenosyl-L-methionine + adenine643 in 25S rRNA | Candida albicans | - |
S-adenosyl-L-homocysteine + N1-methyladenine643 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine643 in 25S rRNA | Candida albicans CBS 356 | - |
S-adenosyl-L-homocysteine + N1-methyladenine643 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine643 in 25S rRNA | Candida albicans RM1000 | - |
S-adenosyl-L-homocysteine + N1-methyladenine643 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine645 in 25S rRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methyladenine645 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine645 in 25S rRNA | Saccharomyces cerevisiae ATCC 204508 | - |
S-adenosyl-L-homocysteine + N1-methyladenine645 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine670 in 25S rRNA | Schizosaccharomyces pombe | - |
S-adenosyl-L-homocysteine + N1-methyladenine670 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine670 in 25S rRNA | Schizosaccharomyces pombe ATCC 24843 | - |
S-adenosyl-L-homocysteine + N1-methyladenine670 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine670 in 25S rRNA | Schizosaccharomyces pombe 972 | - |
S-adenosyl-L-homocysteine + N1-methyladenine670 in 25S rRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
| Candida albicans CBS 356 | - |
- |
- |
| Candida albicans RM1000 | - |
- |
- |
| Saccharomyces cerevisiae | P38961 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | P38961 | - |
- |
| Schizosaccharomyces pombe | Q10257 | - |
- |
| Schizosaccharomyces pombe 972 | Q10257 | - |
- |
| Schizosaccharomyces pombe ATCC 24843 | Q10257 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | 25S/28S rRNA of Candida albicans contains a single m1A methylation in the helix 25.1 | Candida albicans | ? | - |
- |
|
| additional information | 25S/28S rRNA of Schizosaccharomyces pombe contains a single m1A methylation in the helix 25.1 | Schizosaccharomyces pombe | ? | - |
- |
|
| additional information | 25S/28S rRNA of Candida albicans contains a single m1A methylation in the helix 25.1 | Candida albicans CBS 356 | ? | - |
- |
|
| additional information | 25S/28S rRNA of Schizosaccharomyces pombe contains a single m1A methylation in the helix 25.1 | Schizosaccharomyces pombe ATCC 24843 | ? | - |
- |
|
| additional information | 25S/28S rRNA of Schizosaccharomyces pombe contains a single m1A methylation in the helix 25.1 | Schizosaccharomyces pombe 972 | ? | - |
- |
|
| additional information | 25S/28S rRNA of Candida albicans contains a single m1A methylation in the helix 25.1 | Candida albicans RM1000 | ? | - |
- |
|
| S-adenosyl-L-methionine + adenine643 in 25S rRNA | - |
Candida albicans | S-adenosyl-L-homocysteine + N1-methyladenine643 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine643 in 25S rRNA | - |
Candida albicans CBS 356 | S-adenosyl-L-homocysteine + N1-methyladenine643 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine643 in 25S rRNA | - |
Candida albicans RM1000 | S-adenosyl-L-homocysteine + N1-methyladenine643 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine645 in 25S rRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methyladenine645 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine645 in 25S rRNA | - |
Saccharomyces cerevisiae ATCC 204508 | S-adenosyl-L-homocysteine + N1-methyladenine645 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine670 in 25S rRNA | - |
Schizosaccharomyces pombe | S-adenosyl-L-homocysteine + N1-methyladenine670 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine670 in 25S rRNA | - |
Schizosaccharomyces pombe ATCC 24843 | S-adenosyl-L-homocysteine + N1-methyladenine670 in 25S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenine670 in 25S rRNA | - |
Schizosaccharomyces pombe 972 | S-adenosyl-L-homocysteine + N1-methyladenine670 in 25S rRNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5S rRNA (adenine(645)-N(1))-methyltransferase | UniProt | Saccharomyces cerevisiae |
| CaRRP8 | - |
Candida albicans |
| ribosomal RNA-processing protein 8 | UniProt | Candida albicans |
| ribosomal RNA-processing protein 8 | UniProt | Saccharomyces cerevisiae |
| ribosomal RNA-processing protein 8 | UniProt | Schizosaccharomyces pombe |
| Rrp8 | - |
Saccharomyces cerevisiae |
| Rrp8 | - |
Candida albicans |
| Rrp8 | - |
Schizosaccharomyces pombe |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Candida albicans | |
| S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | |
| S-adenosyl-L-methionine | - |
Schizosaccharomyces pombe | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the N1-methyladenosine in helix 25.1 of 25/28S rRNA seems to be highly conserved | Candida albicans |
| evolution | the N1-methyladenosine in helix 25.1 of 25/28S rRNA seems to be highly conserved | Saccharomyces cerevisiae |
| evolution | the N1-methyladenosine in helix 25.1 of 25/28S rRNA seems to be highly conserved | Schizosaccharomyces pombe |
| malfunction | Loss of m1A645 in yeast alters the rRNA topology by affecting eL32 interaction with 25S rRNA. Methylation of the N1 atom of adenosine leads to a net positive charge on the base and is expected to disrupt the canonical Watson-Crick base pairing. The loss of m1A645 leads to structural changes in and around the helix 25.1 region of the 25S rRNA and causes formation of stalled preinitiation complex called halfmers. Loss of m1A645 does not influence the conformation of helix 7 and 60S synthesis. But 60S lacking m1A645 are less competent to bind to 40S subunits and therefore cause the formation of halfmers in the absence of m1A645. Loss of m1A645 in yeast alters the rRNA topology by affecting eL32 interaction with 25S rRNA. Loss of Rrp8 leads to an accumulation of the aberrant 21S pre-rRNA due to defects in A2 cleavage and to cold sensitivity for growth. Both of these phenotypes are fully complemented by Candida albicans CaRRP8, whereas the expression of Schizosaccharomyces pombe SpRRP8 cannot restore 21S accumulation defects and cold sensitivity. The human nucleomethylin (NML or RRP8) fails to complement the pre-rRNA processing phenotype and the growth cold sensitivity of DELTArrp8 | Saccharomyces cerevisiae |
| physiological function | the enzyme CaRRP8 from Candida albicans can fully complement the Saccharomyces cerevisiae rrp8 deficient mutant phenotype, i.e. accumulation of the aberrant 21S pre-rRNA due to defects in A2 cleavage and to cold sensitivity for growth | Candida albicans |
| physiological function | the expression of Schizosaccharomyces pombe SpRRP8 cannot restore 21S accumulation defects and cold sensitivity in of Saccharomyces cerevisiae DELTArrp8 mutant strain | Schizosaccharomyces pombe |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
