Literature summary for 2.1.1.285 extracted from

Freel Meyers, C.L.; Oberth�r, M.; Xu, H.; Heide, L.; Kahne, D.; Walsh, C.T.
Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring (2004), Angew. Chem. Int. Ed. Engl., 43, 67-70.

Search for more literature for 2.1.1.285

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces niveus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0095	-	demethyldecarbamoylnovobiocin	pH 8.5, 22°C	Streptomyces niveus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + demethyldecarbamoylnovobiocin	Streptomyces niveus	the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin	S-adenosyl-L-homocysteine + decarbamoylnovobiocin	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces niveus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
His6-tagged protein	Streptomyces niveus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + demethyldecarbamoylnovobiocin	-	Streptomyces niveus	S-adenosyl-L-homocysteine + decarbamoylnovobiocin	-	?
S-adenosyl-L-methionine + demethyldecarbamoylnovobiocin	the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin	Streptomyces niveus	S-adenosyl-L-homocysteine + decarbamoylnovobiocin	-	?

Synonyms

Synonyms	Comment	Organism
NovP	-	Streptomyces niveus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Streptomyces niveus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.4	-	demethyldecarbamoylnovobiocin	pH 8.5, 22°C	Streptomyces niveus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Streptomyces niveus

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin	Streptomyces niveus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
42.1	-	demethyldecarbamoylnovobiocin	pH 8.5, 22°C	Streptomyces niveus

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Release 2023.1 (January 2023)