Literature summary for 2.1.1.284 extracted from

  • Pacholec, M.; Tao, J.; Walsh, C.T.
    CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis (2005), Biochemistry, 44, 14969-14976.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces niveus

Inhibitors

Inhibitors Comment Organism Structure
additional information no substrate inhibition is observed at 2 mM S-adenosyl-L-methionine Streptomyces niveus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0265
-
8-demethylnovobiocic acid pH 8.5, temperature not specified in the publication Streptomyces niveus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
-
Streptomyces niveus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 8-demethylnovobiocic acid Streptomyces niveus the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin S-adenosyl-L-homocysteine + novobiocic acid
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces niveus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces niveus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 8-demethylnovobiocic acid
-
Streptomyces niveus S-adenosyl-L-homocysteine + novobiocic acid
-
?
S-adenosyl-L-methionine + 8-demethylnovobiocic acid the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin Streptomyces niveus S-adenosyl-L-homocysteine + novobiocic acid
-
?

Synonyms

Synonyms Comment Organism
NovO
-
Streptomyces niveus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
8-demethylnovobiocic acid pH 8.5, temperature not specified in the publication Streptomyces niveus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
Tris-HCl buffer Streptomyces niveus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin Streptomyces niveus