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Literature summary for 2.1.1.284 extracted from
- CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis (2005), Biochemistry, 44, 14969-14976.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces niveus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no substrate inhibition is observed at 2 mM S-adenosyl-L-methionine | Streptomyces niveus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0265 | - |
8-demethylnovobiocic acid | pH 8.5, temperature not specified in the publication | Streptomyces niveus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26500 | - |
- |
Streptomyces niveus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 8-demethylnovobiocic acid | Streptomyces niveus | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin | S-adenosyl-L-homocysteine + novobiocic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces niveus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces niveus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 8-demethylnovobiocic acid | - |
Streptomyces niveus | S-adenosyl-L-homocysteine + novobiocic acid | - |
? | |
| S-adenosyl-L-methionine + 8-demethylnovobiocic acid | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin | Streptomyces niveus | S-adenosyl-L-homocysteine + novobiocic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NovO | - |
Streptomyces niveus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
8-demethylnovobiocic acid | pH 8.5, temperature not specified in the publication | Streptomyces niveus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
Tris-HCl buffer | Streptomyces niveus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin | Streptomyces niveus |
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