Application | Comment | Organism |
---|---|---|
synthesis | O-methyltransferases (OMTs) from microorganism sources have broad scope to methylate diverse classes of plant natural products to synthesize methyl derivatives. Enzyme SpOMT2884 from Streptomyces peucetius cloned into Escherichia coli shows the possibility of generating diverse O-methyl flavonoids 7,8-dihydroxyflavone (7,8-DHF), luteolin, quercetin, rutin, naringenin, daidzein, and formononetin | Streptomyces peucetius subsp. caesius |
Cloned (Comment) | Organism |
---|---|
gene SpOMT7740, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue | Streptomyces peucetius subsp. caesius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | complete inhibition | Streptomyces peucetius subsp. caesius | |
Cu2+ | complete inhibition | Streptomyces peucetius subsp. caesius | |
Zn2+ | complete inhibition | Streptomyces peucetius subsp. caesius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, best cation | Streptomyces peucetius subsp. caesius | |
additional information | no or poor effect by Ca2+, Ni2+, Fe2+, Fe3+, and EDTA | Streptomyces peucetius subsp. caesius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces peucetius subsp. caesius | A0A1B1W6Z7 | - |
- |
Streptomyces peucetius subsp. caesius ATCC 27952 | A0A1B1W6Z7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Streptomyces peucetius subsp. caesius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows a broad substrate specificity. SpOMT7740 catalyzes the O-methylation reaction to form various natural and non-natural O-methoxides, includes 7-hydroxy-8-O-methoxy flavone, 3-O-methoxy flavone, three mono-, di-, and tri-O-methoxy genistein, mono-O-methoxy phloretin, mono-O-methoxy luteolin, 3-O-methoxy beta-sitosterol, and O-methoxy anthraquinones (emodin and aloe emodin) and O-methoxy anthracycline (daunorubicin) exhibiting diverse substrate flexibility. Mass spectrometric analysis of reaction products. 7,8-Dihydroxyflavone is the best substrate for bioconversion to 7-hydroxy-8-O-methoxy flavone. The enzyme seems to prefer methylation of hydroxy groups at C8 position. No or poor activity with doxorubicin and daunorubicin | Streptomyces peucetius subsp. caesius | ? | - |
- |
|
S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone | - |
Streptomyces peucetius subsp. caesius | S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone | - |
? | |
S-adenosyl-L-methionine + 3-hydroxyflavone | lower activity | Streptomyces peucetius subsp. caesius | S-adenosyl-L-homocysteine + 3-methoxyflavone | - |
? | |
S-adenosyl-L-methionine + 7,8-dihydroxyflavone | best substrate | Streptomyces peucetius subsp. caesius | S-adenosyl-L-homocysteine + 7-hydroxy-8-methoxyflavone | - |
? | |
S-adenosyl-L-methionine + aloe emodin | low activity | Streptomyces peucetius subsp. caesius | S-adenosyl-L-homocysteine + 8-methoxy-1-hydroxy-3-(hydroxymethyl)anthracene-9,10-dione | - |
? | |
S-adenosyl-L-methionine + beta-sitosterol | low activity | Streptomyces peucetius subsp. caesius | S-adenosyl-L-homocysteine + 3-methoxy-beta-sitosterol | - |
? | |
S-adenosyl-L-methionine + emodin | low activity | Streptomyces peucetius subsp. caesius | S-adenosyl-L-homocysteine + questin | - |
? | |
S-adenosyl-L-methionine + genistein | when genistein is used as substrate, mass spectras of three O-methoxy genistein (mono-, di-, and tri-O-methoxy genistein) are detected, low activity | Streptomyces peucetius subsp. caesius | S-adenosyl-L-homocysteine + O-methoxy-genistein | - |
? | |
S-adenosyl-L-methionine + luteolin | lower activity | Streptomyces peucetius subsp. caesius | S-adenosyl-L-homocysteine + mono-methoxy-luteolin | - |
? | |
S-adenosyl-L-methionine + phloretin | lower activity | Streptomyces peucetius subsp. caesius | S-adenosyl-L-homocysteine + mono-methoxy-phloretin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, recombinant enzyme, SDS-PAGE | Streptomyces peucetius subsp. caesius |
Synonyms | Comment | Organism |
---|---|---|
CGZ69_28180 | - |
Streptomyces peucetius subsp. caesius |
More | see also 2.1.1.88, cf. EC 2.1.1.42 | Streptomyces peucetius subsp. caesius |
O-methyltransferase | - |
Streptomyces peucetius subsp. caesius |
OMT | - |
Streptomyces peucetius subsp. caesius |
SpOMT7740 | - |
Streptomyces peucetius subsp. caesius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Streptomyces peucetius subsp. caesius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 75 | activity range, recombinant enzyme | Streptomyces peucetius subsp. caesius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | recombinant enzyme | Streptomyces peucetius subsp. caesius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 11.3 | activity range, recombinant enzyme | Streptomyces peucetius subsp. caesius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Streptomyces peucetius subsp. caesius |
General Information | Comment | Organism |
---|---|---|
evolution | O-methyltransferases (OMTs) are categorized into three classes, where class 1 and class 2 are involved in the methylation of phenolic hydroxyl residues and class 3 is involved in the methylation of carboxyl groups to yield methyl esters | Streptomyces peucetius subsp. caesius |
metabolism | methyltransferases are involved in post-modification during biosynthesis of secondary metabolites performing terminal modification | Streptomyces peucetius subsp. caesius |
additional information | O-methyltransferases (OMTs) are commonly regiospecific | Streptomyces peucetius subsp. caesius |