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Literature summary for 2.1.1.28 extracted from

  • Evinger, M.J.; Powers, J.F.; Tischler, A.S.
    Transcriptional silencing of glucocorticoid-inducible phenylethanolamine N-methyltransferase expression by sequential signaling events (2007), Exp. Cell Res., 313, 772-781.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression analysis, transcriptional response of the PNMT gene to GDNF and cpt-cAMP involves distal portions of the PNMT promoter, transient expression of PNMT promoter constructs in MPC 10/9CRC1 cultures Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of PNMT promoter constructs using nested deletion constructs treated with DEX or sequentially with GDNF/cAMP/DEX, the suppression of PNMT induction is reversible Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus the phenylethanolamine N-methyltransferase GRE plays a necessary, but not sufficient role in the transcriptional aspects to the control mechanism. Analyses implicate the participation of sequences in the distal 5´promoter of this gene. ?
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Organism

Organism UniProt Comment Textmining
Mus musculus
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Mus musculus
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mouse pheochromocytoma cell lines 862L and 10/9CRC1 from tumors arising in separate mice with a heterozygous knockout of the neurofibromatosis gene Nf1
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Source Tissue

Source Tissue Comment Organism Textmining
10/9CRC1 cell pheochromocytoma cell line Mus musculus
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862L cell pheochromocytoma cell line Mus musculus
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adrenal gland adrenergic chromaffin cells and medullary tissue Mus musculus
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chromaffin cell phenylethanolamine N-methyltransferase mRNA levels are 30fold increased by dexamethasone. Glial cell line-derived neurotrophic factor and cpt-cAMP each exert negligible influence on baseline phenylethanolamine N-methyltransferase mRNA levels. cAMP effectively blocks dexamethasone induction of phenylethanolamine N-methyltransferase mRNA. Mus musculus
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pheochromocytoma cell pheochromocytomas are tumors of adrenal chromaffin cells. Phenylethanolamine N-methyltransferase mRNA levels are markedly increased by glucocorticoid administration. Dexamethasone elicites approximately 800fold increases in phenylethanolamine N-methyltransferase mRNA levels in mouse pheochromocytoma 862L cells and approximately 75fold increases for mouse pheochromocytoma 10/9CR1 cells. Glial cell line-derived neurotrophic factor and cpt-cAMP alone each produce little or no change in baseline phenylethanolamine N-methyltransferase mRNA levels. In both cell lines, dexamethasone-stimulated increases in phenylethanolamine N-methyltranferase mRNA levels are reduced approximately 90% by prior tratment with cAMP. Glial cell line-derived neurotrophic factor reduces dexamethasone effects on phenylethanolamine N-methyltransferase mRNA levels by 40% in mouse pheochromocytoma 862L cells, while not supressing the dexamethasone response in mouse pheochromocytoma 10/9CRC1 cells. Pretreatment with cAMP and afterwards treatment with glial cell line-derived neurotrophic factor before additon of dexamethasone reduced phenylethanolamine N-methyltransferase expression in mouse pheochromocytoma 862L and 10/9CRC1 cells to 0.1% and 5.5% of their corresponding DEX induced maxima. Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the phenylethanolamine N-methyltransferase GRE plays a necessary, but not sufficient role in the transcriptional aspects to the control mechanism. Analyses implicate the participation of sequences in the distal 5´promoter of this gene. Mus musculus ?
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?

Synonyms

Synonyms Comment Organism
PNMT
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Mus musculus