Cloned (Comment) | Organism |
---|---|
expression analysis, transcriptional response of the PNMT gene to GDNF and cpt-cAMP involves distal portions of the PNMT promoter, transient expression of PNMT promoter constructs in MPC 10/9CRC1 cultures | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of PNMT promoter constructs using nested deletion constructs treated with DEX or sequentially with GDNF/cAMP/DEX, the suppression of PNMT induction is reversible | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | the phenylethanolamine N-methyltransferase GRE plays a necessary, but not sufficient role in the transcriptional aspects to the control mechanism. Analyses implicate the participation of sequences in the distal 5´promoter of this gene. | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus | - |
mouse pheochromocytoma cell lines 862L and 10/9CRC1 from tumors arising in separate mice with a heterozygous knockout of the neurofibromatosis gene Nf1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
10/9CRC1 cell | pheochromocytoma cell line | Mus musculus | - |
862L cell | pheochromocytoma cell line | Mus musculus | - |
adrenal gland | adrenergic chromaffin cells and medullary tissue | Mus musculus | - |
chromaffin cell | phenylethanolamine N-methyltransferase mRNA levels are 30fold increased by dexamethasone. Glial cell line-derived neurotrophic factor and cpt-cAMP each exert negligible influence on baseline phenylethanolamine N-methyltransferase mRNA levels. cAMP effectively blocks dexamethasone induction of phenylethanolamine N-methyltransferase mRNA. | Mus musculus | - |
pheochromocytoma cell | pheochromocytomas are tumors of adrenal chromaffin cells. Phenylethanolamine N-methyltransferase mRNA levels are markedly increased by glucocorticoid administration. Dexamethasone elicites approximately 800fold increases in phenylethanolamine N-methyltransferase mRNA levels in mouse pheochromocytoma 862L cells and approximately 75fold increases for mouse pheochromocytoma 10/9CR1 cells. Glial cell line-derived neurotrophic factor and cpt-cAMP alone each produce little or no change in baseline phenylethanolamine N-methyltransferase mRNA levels. In both cell lines, dexamethasone-stimulated increases in phenylethanolamine N-methyltranferase mRNA levels are reduced approximately 90% by prior tratment with cAMP. Glial cell line-derived neurotrophic factor reduces dexamethasone effects on phenylethanolamine N-methyltransferase mRNA levels by 40% in mouse pheochromocytoma 862L cells, while not supressing the dexamethasone response in mouse pheochromocytoma 10/9CRC1 cells. Pretreatment with cAMP and afterwards treatment with glial cell line-derived neurotrophic factor before additon of dexamethasone reduced phenylethanolamine N-methyltransferase expression in mouse pheochromocytoma 862L and 10/9CRC1 cells to 0.1% and 5.5% of their corresponding DEX induced maxima. | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the phenylethanolamine N-methyltransferase GRE plays a necessary, but not sufficient role in the transcriptional aspects to the control mechanism. Analyses implicate the participation of sequences in the distal 5´promoter of this gene. | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PNMT | - |
Mus musculus |