BRENDA - Enzyme Database show
show all sequences of 2.1.1.279

Biosynthesis of estragole and t-anethole in bitter fennel (Foeniculum vulgare Mill. var. vulgare) chemotypes. Changes in SAM:phenylpropene O-methyltransferase activities during development

Gross, M.; Friedman, J.; Dudai, N.; Larkov, O.; Cohen, Y.; Bar, E.; Ravid, U.; Putievsky, E.; Lewinsohn, E.; Plant Sci. 163, 1047-1053 (2002)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
S-adenosyl-L-methionine
in 50 mM bis-Tris pH 7.0, at 30°C
Foeniculum vulgare
0.032
-
trans-anol
in 50 mM bis-Tris pH 7.0, at 30°C
Foeniculum vulgare
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
x * 80000, SDS-PAGE
Foeniculum vulgare
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + trans-anol
Foeniculum vulgare
-
S-adenosyl-L-homocysteine + trans-anethole
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Foeniculum vulgare
-
-
-
Purification (Commentary)
Commentary
Organism
Sephadex G-25 gel filtration
Foeniculum vulgare
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
highest expression
Foeniculum vulgare
-
fruit
-
Foeniculum vulgare
-
leaf
-
Foeniculum vulgare
-
stem
lowest expression
Foeniculum vulgare
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + trans-anol
-
726235
Foeniculum vulgare
S-adenosyl-L-homocysteine + trans-anethole
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 80000, SDS-PAGE
Foeniculum vulgare
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Foeniculum vulgare
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
45
he reaction rate increases with temperature up to 25°C, remains roughly constant till reaching 37°C and at 45°C activity dramatically decreases
Foeniculum vulgare
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
S-adenosyl-L-methionine
in 50 mM bis-Tris pH 7.0, at 30°C
Foeniculum vulgare
0.032
-
trans-anol
in 50 mM bis-Tris pH 7.0, at 30°C
Foeniculum vulgare
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
x * 80000, SDS-PAGE
Foeniculum vulgare
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + trans-anol
Foeniculum vulgare
-
S-adenosyl-L-homocysteine + trans-anethole
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Sephadex G-25 gel filtration
Foeniculum vulgare
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
highest expression
Foeniculum vulgare
-
fruit
-
Foeniculum vulgare
-
leaf
-
Foeniculum vulgare
-
stem
lowest expression
Foeniculum vulgare
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + trans-anol
-
726235
Foeniculum vulgare
S-adenosyl-L-homocysteine + trans-anethole
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 80000, SDS-PAGE
Foeniculum vulgare
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Foeniculum vulgare
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
45
he reaction rate increases with temperature up to 25°C, remains roughly constant till reaching 37°C and at 45°C activity dramatically decreases
Foeniculum vulgare
Expression
Organism
Commentary
Expression
Foeniculum vulgare
in developing fruits, enzyme activity levels increase until the wasty stage is reached and then dramatically decrease. Young leaves display higher levels of enzyme activity than old leaves
up
Expression (protein specific)
Organism
Commentary
Expression
Foeniculum vulgare
in developing fruits, enzyme activity levels increase until the wasty stage is reached and then dramatically decrease. Young leaves display higher levels of enzyme activity than old leaves
up
Other publictions for EC 2.1.1.279
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700822
Koeduka
Biosynthesis of t-anethole in ...
Pimpinella anisum
Plant Physiol.
149
384-394
2009
-
-
1
-
-
-
5
2
-
-
-
-
-
1
-
-
-
2
-
5
-
-
5
-
-
-
3
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
-
-
5
-
-
5
-
-
-
3
1
1
1
-
-
-
-
-
-
1
1
726235
Gross
-
Biosynthesis of estragole and ...
Foeniculum vulgare
Plant Sci.
163
1047-1053
2002
-
-
-
-
-
-
-
2
-
-
1
1
-
1
-
-
1
-
-
4
-
-
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
4
-
-
1
1
1
1
-
-
-
-
-
-
1
-
-
1
-
-