Any feedback?
Literature summary for 2.1.1.278 extracted from
- Structural, biochemical, and phylogenetic analyses suggest that indole-3-acetic acid methyltransferase is an evolutionarily ancient member of the SABATH family (2008), Plant Physiol., 146, 455-467.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Oryza sativa Japonica Group |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the crystal structure of the enzyme is determined and refined to 2.75 A resolution | Arabidopsis thaliana |
| the crystal structure of the enzyme is determined and refined to 2.75 A resolution | Oryza sativa Japonica Group |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 15% inhibition | Oryza sativa Japonica Group |  |
| Cu2+ | 5 mM, more than 95% inhibition | Oryza sativa Japonica Group | |
| Fe2+ | 5 mM, more than 95% inhibition | Oryza sativa Japonica Group | |
| Fe3+ | 5 mM, more than 95% inhibition | Oryza sativa Japonica Group | |
| Mg2+ | 5 mM, 15% inhibition | Oryza sativa Japonica Group | |
| Mn2+ | 5 mM, more than 95% inhibition | Oryza sativa Japonica Group | |
| Zn2+ | 5 mM, more than 95% inhibition | Oryza sativa Japonica Group | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0073 | - |
S-adenosyl-L-methionine | pH 7.5, 25°C | Oryza sativa Japonica Group | |
| 0.0179 | - |
(indol-3-yl)acetate | pH 7.5, 25°C | Oryza sativa Japonica Group | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 5 mM, more than 1.5fold stimulation | Oryza sativa Japonica Group | |
| Na+ | 5 mM, more than 1.5fold stimulation | Oryza sativa Japonica Group | |
| NH4+ | 5 mM, more than 1.5fold stimulation | Oryza sativa Japonica Group | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43800 | - |
x * 43800, calculated from sequence | Oryza sativa Japonica Group |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + (indol-3-yl)acetate | Arabidopsis thaliana | the enzyme modulates homeostasis of (indol-3-yl)acetate in plant tissues through methylation of the free carboxyl group of (indol-3-yl)acetate | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? | |
| S-adenosyl-L-methionine + (indol-3-yl)acetate | Oryza sativa Japonica Group | the enzyme modulates homeostasis of (indol-3-yl)acetate in plant tissues through methylation of the free carboxyl group of (indol-3-yl)acetate | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9FLN8 | - |
- |
| Oryza sativa Japonica Group | Q0J998 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryza sativa Japonica Group |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with: salicylate, jasmonic acid, farnesoic acid and gibberellic acid | Oryza sativa Japonica Group | ? | - |
? | |
| S-adenosyl-L-methionine + (indol-3-yl)acetate | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? | |
| S-adenosyl-L-methionine + (indol-3-yl)acetate | - |
Oryza sativa Japonica Group | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? | |
| S-adenosyl-L-methionine + (indol-3-yl)acetate | the enzyme modulates homeostasis of (indol-3-yl)acetate in plant tissues through methylation of the free carboxyl group of (indol-3-yl)acetate | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? | |
| S-adenosyl-L-methionine + (indol-3-yl)acetate | the enzyme modulates homeostasis of (indol-3-yl)acetate in plant tissues through methylation of the free carboxyl group of (indol-3-yl)acetate | Oryza sativa Japonica Group | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? | |
| S-adenosyl-L-methionine + (indol-3-yl)butyrate | at 2% of the catalytic activity compared to (indol-3-yl)acetate | Oryza sativa Japonica Group | S-adenosyl-L-homocysteine + methyl (indol-3-yl)butyrate | - |
? | |
| S-adenosyl-L-methionine + dichlorophenoxyacetic acid | at 5% of the catalytic activity compared to (indol-3-yl)acetate | Oryza sativa Japonica Group | S-adenosyl-L-homocysteine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43800, calculated from sequence | Oryza sativa Japonica Group |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtIAMT1 | - |
Arabidopsis thaliana |
| IAA methyltransferase | - |
Arabidopsis thaliana |
| IAA methyltransferase | - |
Oryza sativa Japonica Group |
| indole-3-acetic acid methyltransferase | - |
Arabidopsis thaliana |
| indole-3-acetic acid methyltransferase | - |
Oryza sativa Japonica Group |
| OsIAMT1 | - |
Oryza sativa Japonica Group |
| OsSABATH4 | - |
Oryza sativa Japonica Group |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
S-adenosyl-L-methionine | pH 7.5, 25°C | Oryza sativa Japonica Group | |
| 0.025 | - |
(indol-3-yl)acetate | pH 7.5, 25°C | Oryza sativa Japonica Group | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Oryza sativa Japonica Group |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: 20% of maximal activity, pH 9.0: 30% of maximal activity | Oryza sativa Japonica Group |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
