Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) CodonPlus cells | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 65% residual activity at 5 mM | Glycine max | |
Cu2+ | complete inhibition at 5 mM | Glycine max | |
Fe2+ | complete inhibition at 5 mM | Glycine max | |
Fe3+ | complete inhibition at 5 mM | Glycine max | |
Mn2+ | about 25% residual activity at 5 mM | Glycine max | |
Zn2+ | complete inhibition at 5 mM | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00046 | - |
salicylate | in 50 mM Tris-HCl, at pH 7.5 and 25°C | Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 2fold increase in activity in the presence of 5 mM K+ | Glycine max | |
additional information | Na+, NH4+ and Mg2+ have no effect on isoform SAMT1 activity | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Glycine max | jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1 | ? | - |
? | |
S-adenosyl-L-methionine + 3-hydroxybenzoate | Glycine max | 1.8% activity compared to salicylate | S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate | - |
? | |
S-adenosyl-L-methionine + anthranilate | Glycine max | 9.3% activity compared to salicylate | ? | - |
? | |
S-adenosyl-L-methionine + benzoate | Glycine max | 16.9% activity compared to salicylate | ? | - |
? | |
S-adenosyl-L-methionine + salicylate | Glycine max | highest level of specific activity with salicylate | S-adenosyl-L-homocysteine + methyl salicylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hairy root | - |
Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1 | Glycine max | ? | - |
? | |
S-adenosyl-L-methionine + 3-hydroxybenzoate | 1.8% activity compared to salicylate | Glycine max | S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate | - |
? | |
S-adenosyl-L-methionine + anthranilate | 9.3% activity compared to salicylate | Glycine max | ? | - |
? | |
S-adenosyl-L-methionine + benzoate | 16.9% activity compared to salicylate | Glycine max | ? | - |
? | |
S-adenosyl-L-methionine + salicylate | highest level of specific activity with salicylate | Glycine max | S-adenosyl-L-homocysteine + methyl salicylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
salicylic acid methyltransferase | - |
Glycine max |
SAMT1 | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Glycine max |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | about 80% activity at 10°C, about 90% activity at 20°C, about 78% activity at 30°C, about 70% activity at 40°C | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | about 90% activity at pH 7.0, about 40% activity at pH 8.0 | Glycine max |
General Information | Comment | Organism |
---|---|---|
physiological function | isoform SAM1 plays a role in soybean defence against the soybean cyst nematode Heterodera glycines. Enzyme overexpression also affects the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction | Glycine max |