Cloned (Comment) | Organism |
---|---|
subcloned into expression vector pET-28a, containing an N-terminal polyhistidine 6-His-tag, and expressed in Escherichia coli | Antirrhinum majus |
General Stability | Organism |
---|---|
the enzyme is active in both Tris- and phosphatecitrate-based buffers, although its activity in phosphate-citrate buffer is about 20% lower than in Tris-buffer | Antirrhinum majus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, strong inhibitory effect with more than 95% inhibition | Antirrhinum majus | |
Fe2+ | 5 mM, strong inhibitory effect with more than 95% inhibition | Antirrhinum majus | |
Mn2+ | 5 mM, inhibits SAMT activity less than 30% | Antirrhinum majus | |
additional information | presence of 5 mM Mg2+ in the assay reaction and addition of K+, NH4+ and Ca2+ does not affect SAMT activity | Antirrhinum majus | |
Na+ | 5 mM, inhibits SAMT activity less than 30% | Antirrhinum majus | |
Zn2+ | 5 mM, inhibits SAMT activity less than 30% | Antirrhinum majus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
S-adenosyl-L-methionine | with salicylic acid, pH 7.5, 20°C | Antirrhinum majus | |
0.083 | - |
salicylic acid | pH 7.5, 20°C | Antirrhinum majus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
SDS-PAGE | Antirrhinum majus |
107000 | - |
with a calibrated Sephacryl 200-HR column | Antirrhinum majus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Antirrhinum majus | Q8H6N2 | snapdragon | - |
Purification (Comment) | Organism |
---|---|
by nickel-based affinity chromatography | Antirrhinum majus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
petal | 200fold higher levels of SAMT mRNA than in the pistil, where lowest level of mRNA expression is observed | Antirrhinum majus | - |
sepal | Expression of SAMT mRNA about half the level in petal, where lowest level of mRNA expression is observed | Antirrhinum majus | - |
stamen | expression of SAMT only 2fold higher than in pistil, where lowest level of mRNA expression is observed | Antirrhinum majus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000126 | - |
salicylic acid, pH 7.5, 20°C | Antirrhinum majus |
Storage Stability | Organism |
---|---|
the recombinant protein is catalytically stable for more than a year when stored at -80°C | Antirrhinum majus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is also active with benzoic acid resulting in methyl benzoate formation | Antirrhinum majus | ? | - |
? | |
additional information | no activity with 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, trans-cinnamic acid, p-coumaric acid, m-coumaric acid, o-coumaric acid and benzyl alcohol | Antirrhinum majus | ? | - |
? | |
S-adenosyl-L-methionine + salicylic acid | - |
Antirrhinum majus | S-adenosyl-L-homocysteine + methyl salicylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 49000, SDS-PAGE | Antirrhinum majus |
Synonyms | Comment | Organism |
---|---|---|
salicylic acid carboxyl methyltransferase | - |
Antirrhinum majus |
SAMT | - |
Antirrhinum majus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
enzyme is stable at temperatures up to 42°C for 30 min but 30-min incubation at 65°C leads to a 70% loss of activity | Antirrhinum majus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
salicylic acid | pH 7.5, 20°C | Antirrhinum majus | |
0.013 | - |
S-adenosyl-L-methionine | with salicylic acid, pH 7.5, 20°C | Antirrhinum majus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Antirrhinum majus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
at pH 4.0 and 9.5 the enzyme activity falls to about 20% of the maximal activity | Antirrhinum majus |
5 | 8 | with 8090% of maximum activity | Antirrhinum majus |