Cloned (Comment) | Organism |
---|---|
gene SABATH24, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overview, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Populus trichocarpa |
Protein Variants | Comment | Organism |
---|---|---|
H157M | site-directed mutagenesis, this mutation results in a switch from a preference for benzoic acid (BA) over salicylic acid (SA) in wild-type PtSABATH24 to a preference for SA over BA in the H157M mutant | Populus trichocarpa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + benzoate | Populus trichocarpa | - |
methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + salicylate | Populus trichocarpa | - |
methyl salicylate + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | B9IPD3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Populus trichocarpa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
56.97 | - |
recombinant wild-type PtSABATH24 with benzoic acid, pH 7.4, 25°C | Populus trichocarpa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme PtSABATH24 has a wide substrate spectrum, exhibiting enzymatic activity towards eight of the substrates tested, i.e. indole-3-acetic acid, benzoic acid, salicylic acid, vanillic acid, farnesoic acid, nicotinic acid, coumalic acid, and trans-cinnamic acid, with the highest enzymatic activity towards benzoic acid. Compared with other Populus SABATH proteins, PtSABATH24 shows at least 42.5fold higher enzymatic activity towards benzoic acid | Populus trichocarpa | ? | - |
- |
|
S-adenosyl-L-methionine + benzoate | - |
Populus trichocarpa | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + salicylate | - |
Populus trichocarpa | methyl salicylate + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class II SABATH protein | - |
Populus trichocarpa |
More | see also EC 2.1.1.274 | Populus trichocarpa |
PtSABATH24 | - |
Populus trichocarpa |
S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase | UniProt | Populus trichocarpa |
SABATH methyltransferase 4 | UniProt | Populus trichocarpa |
SABATH24 | - |
Populus trichocarpa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Populus trichocarpa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Populus trichocarpa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Populus trichocarpa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the SABATH family, phylogenetic analysis and tree, detailed overview. Twenty-eight Populus SABATH genes are divided into three classes with distinct divergences in their gene structure, expression responses to abiotic stressors and enzymatic properties of encoded proteins. Populus class I SABATH proteins convert indole-3-acetic acid (IAA) to methyl-IAA, class II SABATH proteins convert benzoic acid (BA) and salicylic acid (SA) to methyl-BA and methyl-SA, while class III SABATH proteins convert farnesoic acid (FA) to methyl-FA. For Populus class II SABATH proteins, both forward and reverse mutagenesis studies show that a single amino acid switch between PtSABATH4 and PtSABATH24 results in substrate switch. Of the Populus SABATH class II proteins, PtSABATH4 and 24 show the highest activity towards SA and BA, respectively | Populus trichocarpa |
malfunction | for Populus class II SABATH proteins, both forward and reverse mutagenesis studies show that a single amino acid switch between PtSABATH4 and PtSABATH24 results in substrate switch. Mutation of His157 of PtSABATH24 to a methionine residue also results in a switch from a preference for BA over SA in wild-type PtSABATH24 to a preference for SA over BA in the H157M mutant. The mutation M156H in PtSBATH4 (EC 2.1.1.274) results in a switch from a preference for salicylic acid (SA) over benzoic acid (BA) in wild-type PtSABATH4 to a preference for BA over SA in the M156H mutant | Populus trichocarpa |
metabolism | expression patterns of Populus SABATH genes under normal growth conditions and abiotic stress, overview | Populus trichocarpa |