Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.272 extracted from

  • Moore, S.J.; Biedendieck, R.; Lawrence, A.D.; Deery, E.; Howard, M.J.; Rigby, S.E.; Warren, M.J.
    Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway (2013), J. Biol. Chem., 288, 297-305.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.272

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus megaterium DSM319 Priestia megaterium

Protein Variants

Protein Variants Comment Organism
C402A mutation introduced to disrupt the iron-sulfur center. Mutant lacks any visible coloration and has decreased absorbance between 350 and 420 nm and less than 95% of wild-type activity Priestia megaterium
C443A mutation introduced to disrupt the iron-sulfur center. Mutant lacks any visible coloration and has decreased absorbance between 350 and 420 nm and less than 95% of wild-type activity Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE of recombinant enzyme including His-tag Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium O87689
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes both methylation at C-17 and ring contraction Priestia megaterium ?
-
 ?
S-adenosyl-L-methionine + cobalt(II)-factor-III + dithiothreitol
-
 Priestia megaterium S-adenosyl-L-homocysteine + cobalt(II)-precorrin-4 + oxidized dithiothreitol
-
 ?
S-adenosyl-L-methionine + cobalt(II)-precorrin-3
-
 Priestia megaterium S-adenosyl-L-homocysteine + cobalt(II)-factor-IV
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE of recombinant enzyme including His-tag Priestia megaterium

Synonyms

Synonyms Comment Organism
CbiH60
-
 Priestia megaterium
Precorrin-3 methylase
-
 Priestia megaterium

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center enzyme contains a [4Fe-4S]-center required for ring contraction reaction. Iron content is 4.8 mol of iron/mol of enzyme Priestia megaterium