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Literature summary for 2.1.1.267 extracted from

  • Okitsu, N.; Mizuno, T.; Matsui, K.; Choi, S.H.; Tanaka, Y.
    Molecular cloning of flavonoid biosynthetic genes and biochemical characterization of anthocyanin O-methyltransferase of Nemophila menziesii Hook. and Arn (2018), Plant Biotechnol., 35, 9-16 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene NmAMT6, phylogenetic tree, functional recombinant expression in Escherichia coli Nemophila menziesii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3'-methoxydelphinidin 3-O-glucoside Nemophila menziesii very low activity, with delphinidin 3-O-glucoside as substrate, NmAMT6 almost exclusively yields petunidin 3-O-glucoside rather than malvidin 3-O-glucoside. This specificity is consistent with the anthocyanin composition of Nemophila petals S-adenosyl-L-homocysteine + 3',5'-dimethoxydelphinidin 3-O-glucoside
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 ?
S-adenosyl-L-methionine + delphinidin 3-O-glucoside Nemophila menziesii
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 S-adenosyl-L-homocysteine + 3'-methoxydelphinidin 3-O-glucoside
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 ?
S-adenosyl-L-methionine + delphinidin 3-O-glucoside Nemophila menziesii with delphinidin 3-O-glucoside as substrate, NmAMT6 almost exclusively yields petunidin 3-O-glucoside rather than malvidin 3-O-glucoside. This specificity is consistent with the anthocyanin composition of Nemophila petals S-adenosyl-L-homocysteine + 3'-methoxydelphinidin 3-O-glucoside
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 ?

Organism

Organism UniProt Comment Textmining
Nemophila menziesii A0A292GLW9
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-

Source Tissue

Source Tissue Comment Organism Textmining
flower developmental flavonoid analysis of Nemophila menziesii flowers, overview Nemophila menziesii
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leaf
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 Nemophila menziesii
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petal
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 Nemophila menziesii
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, overview Nemophila menziesii ?
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-
S-adenosyl-L-methionine + 3'-methoxydelphinidin 3-O-glucoside very low activity, with delphinidin 3-O-glucoside as substrate, NmAMT6 almost exclusively yields petunidin 3-O-glucoside rather than malvidin 3-O-glucoside. This specificity is consistent with the anthocyanin composition of Nemophila petals Nemophila menziesii S-adenosyl-L-homocysteine + 3',5'-dimethoxydelphinidin 3-O-glucoside
-
 ?
S-adenosyl-L-methionine + delphinidin 3-O-glucoside
-
 Nemophila menziesii S-adenosyl-L-homocysteine + 3'-methoxydelphinidin 3-O-glucoside
-
 ?
S-adenosyl-L-methionine + delphinidin 3-O-glucoside with delphinidin 3-O-glucoside as substrate, NmAMT6 almost exclusively yields petunidin 3-O-glucoside rather than malvidin 3-O-glucoside. This specificity is consistent with the anthocyanin composition of Nemophila petals Nemophila menziesii S-adenosyl-L-homocysteine + 3'-methoxydelphinidin 3-O-glucoside
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 ?

Synonyms

Synonyms Comment Organism
anthocyanin methyltransferase UniProt Nemophila menziesii
anthocyanin O-methyltransferase
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 Nemophila menziesii
NmAMT6
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 Nemophila menziesii

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Nemophila menziesii

General Information

General Information Comment Organism
metabolism spatiotemporal transcriptome analysis indicates that genes involved in the early part of the flavonoid biosynthetic pathway are strongly expressed during early-petal development and that those in the late part at late-flower opening stages, but they are rarely expressed in leaves Nemophila menziesii
physiological function the enzyme is involved in biosynthesis of the blue flower color of Nemophila menziesii, which is derived from a metalloanthocyanin, nemophilin. Nemophilin comprises petunidin-3-O-[6-O-(trans-4-coumaroyl)-beta-glucoside]-5-O-[6-O-(malonyl)-beta-glucoside], apigenin-7-O-beta-glucoside-4'-O-(6-O-malonyl)-beta-glucoside, and Mg2+ and Fe3+ ions Nemophila menziesii