Literature summary for 2.1.1.267 extracted from

Nakamura, N.; Katsumoto, Y.; Brugliera, F.; Demelis, L.; Nakajima, D.; Suzuki, H.; Tanaka, Y.
Flower color modification in Rosa hybrida by expressing the S-adenosylmethionine anthocyanin 3',5'-O-methyltransferase gene from Torenia hybrida (2015), Plant Biotechnol., 32, 109-117 .

No PubMed abstract available

Search for more literature for 2.1.1.267

Cloned(Commentary)

Cloned (Comment)	Organism
gene AOMT, cloned from a cDNA library derived from Torenia hybrida petals that mainly accumulated malvidin type anthocyanins using the petunia A3'OMT cDNA as a probe, phylogenetic analysis and tree. Functional recombinant expression in Nierembergia sp. NB18, the petals of which naturally accumulate anthocyanins derived from delphinidin. The resultant transgenic petals produce methylated anthocyanins, based on malvidin and petunidin, in addition to delphinidin. Transgenic rose petals of Rosa hybrida cv. WKS124 expressing both a pansy flavonoid 3',5'-hydroxylase (F3'5'H) and the Torenia A3'5'OMT genes accumulated methylated anthocyanins based upon malvidin, petunidin, and peonidin, which comprise up to 88% of the total anthocyanidins, and their magenta color is more brilliant than that of the petals that accumulate delphinidin type anthocyanins by expressing the F3'5'H gene alone. Agrobacterium tumefaciens strain AGL0 transfection method. Recombinant expression of His-tagged enzyme in Escherichia coli	Torenia hybrid cultivar

Cloned (Comment)

Organism

gene AOMT, cloned from a cDNA library derived from Torenia hybrida petals that mainly accumulated malvidin type anthocyanins using the petunia A3'OMT cDNA as a probe, phylogenetic analysis and tree. Functional recombinant expression in Nierembergia sp. NB18, the petals of which naturally accumulate anthocyanins derived from delphinidin. The resultant transgenic petals produce methylated anthocyanins, based on malvidin and petunidin, in addition to delphinidin. Transgenic rose petals of Rosa hybrida cv. WKS124 expressing both a pansy flavonoid 3',5'-hydroxylase (F3'5'H) and the Torenia A3'5'OMT genes accumulated methylated anthocyanins based upon malvidin, petunidin, and peonidin, which comprise up to 88% of the total anthocyanidins, and their magenta color is more brilliant than that of the petals that accumulate delphinidin type anthocyanins by expressing the F3'5'H gene alone. Agrobacterium tumefaciens strain AGL0 transfection method. Recombinant expression of His-tagged enzyme in Escherichia coli

Torenia hybrid cultivar

Protein Variants

Protein Variants	Comment	Organism
additional information	flower color modification in Rosa hybrida by expressing the S-adenosylmethionine: anthocyanin 3',5'-O-methyltransferase gene from Torenia hybrida, phenotypes of T-DNA insertion transgenic plants, overview	Torenia hybrid cultivar

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0659	-	cyanidin 3-O-glucoside	pH 7.5, 37°C, recombinant enzyme	Torenia hybrid cultivar
0.089	-	delphinidin 3,5-O-diglucoside	pH 7.5, 37°C, recombinant enzyme	Torenia hybrid cultivar
0.103	-	delphinidin 3-O-glucoside	pH 7.5, 37°C, recombinant enzyme	Torenia hybrid cultivar
0.123	-	cyanidin 3,5-O-diglucoside	pH 7.5, 37°C, recombinant enzyme	Torenia hybrid cultivar

Organism

Organism	UniProt	Comment	Textmining
Torenia hybrid cultivar	A0A090BN67	cv. Summerwave Blue	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography	Torenia hybrid cultivar

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	-	Torenia hybrid cultivar	-
petal	-	Torenia hybrid cultivar	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
S-adenosyl-L-methionine + cyanidin 3,5-O-diglucoside	-	Torenia hybrid cultivar	S-adenosyl-L-homocysteine + peonidin 3,5-O-diglucoside	-	?
S-adenosyl-L-methionine + cyanidin 3-O-glucoside	-	Torenia hybrid cultivar	S-adenosyl-L-homocysteine + peonidin 3-O-glucoside	-	?
S-adenosyl-L-methionine + cyanidin 3-O-glucoside-5-O-coumaroylglucoside	-	Torenia hybrid cultivar	S-adenosyl-L-homocysteine + peonidin 3-O-glucoside-5-O-coumaroylglucoside	-	?
S-adenosyl-L-methionine + delphinidin 3,5-O-diglucoside	-	Torenia hybrid cultivar	S-adenosyl-L-homocysteine + petunidin 3,5-O-diglucoside	-	?
S-adenosyl-L-methionine + delphinidin 3-O-glucoside	-	Torenia hybrid cultivar	S-adenosyl-L-homocysteine + petunidin 3-O-glucoside	-	?
S-adenosyl-L-methionine + delphinidin 3-O-glucoside-5-O-coumaroylglucoside	-	Torenia hybrid cultivar	S-adenosyl-L-homocysteine + petunidin 3-O-glucoside-5-O-coumaroylglucoside	-	?
S-adenosyl-L-methionine + petunidin 3,5-O-diglucoside	-	Torenia hybrid cultivar	S-adenosyl-L-homocysteine + malvidin 3,5-O-diglucoside	-	?
S-adenosyl-L-methionine + petunidin 3-O-glucoside	-	Torenia hybrid cultivar	S-adenosyl-L-homocysteine + malvidin 3-O-glucoside	-	?
S-adenosyl-L-methionine + petunidin 3-O-glucoside-5-O-coumaroylglucoside	-	Torenia hybrid cultivar	S-adenosyl-L-homocysteine + malvidin 3-O-glucoside-5-O-coumaroylglucoside	-	?

Synonyms

Synonyms	Comment	Organism
A3'5'OMT	-	Torenia hybrid cultivar
AOMT	-	Torenia hybrid cultivar
S-adenosylmethionine: anthocyanin 3'5'-methyltransferase	UniProt	Torenia hybrid cultivar
ThAOMT	-	Torenia hybrid cultivar
TMT5	-	Torenia hybrid cultivar

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Torenia hybrid cultivar

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Torenia hybrid cultivar

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Torenia hybrid cultivar

General Information

General Information	Comment	Organism
evolution	the torenia A3'5'OMT shares 52-72% amino acid sequence identity with other AOMTs and belongs to the group A1 methyltransferase family that also includes caffeoyl CoA O-methyltransferase	Torenia hybrid cultivar
metabolism	partial overview over the anthocyanin biosynthetic pathway of Torenia hybrida	Torenia hybrid cultivar
additional information	anthocyanidin analysis in Nierembergia petals expressing the Torenia A3'5'OMT gene, overview	Torenia hybrid cultivar