Cloned (Comment) | Organism |
---|---|
gene rlmK, expression in Escherichia coli DELTArlmKL mutant | Neisseria meningitidis |
gene rlmL, renamed rlmKL, encodes a fused methyltransferase responsible for forming both m7G2069 and m2G2445 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D195A | site-directed mutagenesis, the mutation does not affect the methylation activity | Escherichia coli |
D568A | site-directed mutagenesis, the D568A mutation in the C-terminal domain does not rescue m7G2069 formation, but m2G2445 is efficiently formed in this mutant | Escherichia coli |
D597A | site-directed mutagenesis, the mutation does not affect the methylation activity | Escherichia coli |
N309A | site-directed mutagenesis, the N309A mutation in the NTD impairs m2G2445 formation, but rescues m7G2069 formation | Escherichia coli |
N397A | site-directed mutaagenesis, the N397A mutant exhibits no m2G2445 formation but rescues m7G2069 formation | Escherichia coli |
R530A | site-directed mutagenesis, the mutation does not affect the methylation activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme also catalyzes the N2-methylation of guanine2445 in 23S rRNA, RlmL activity, reaction of EC 2.1.1.173 | ? | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | Escherichia coli | RlmK activity in helix 74 of Escherichia coli 23S rRNA | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | Neisseria meningitidis | RlmK activity in helix 74 of Escherichia coli 23S rRNA | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | Neisseria meningitidis MC58 | RlmK activity in helix 74 of Escherichia coli 23S rRNA | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene rlmL, renamed rlmKL | - |
Neisseria meningitidis | Q9JYY8 | gene rlmK | - |
Neisseria meningitidis MC58 | Q9JYY8 | gene rlmK | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also catalyzes the N2-methylation of guanine2445 in 23S rRNA, RlmL activity, reaction of EC 2.1.1.173 | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | RlmK activity in helix 74 of Escherichia coli 23S rRNA | Escherichia coli | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | RlmK activity in helix 74 of Escherichia coli 23S rRNA | Neisseria meningitidis | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine2069 in 23S rRNA | RlmK activity in helix 74 of Escherichia coli 23S rRNA | Neisseria meningitidis MC58 | S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rlmK | - |
Escherichia coli |
rlmK | - |
Neisseria meningitidis |
RlmKL | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | Neisseria meningitidis rlmL and rlmK homologues rescue m2G2445 and m7G2069 formation, respectively, in the Escherichia coli DELTArlmKL strain | Escherichia coli |
malfunction | Neisseria meningitidis rlmL and rlmK homologues rescue m2G2445 and m7G2069 formation, respectively, in the Escherichia coli DELTArlmKL strain, overview | Neisseria meningitidis |
physiological function | rlmKL encodes a fused methyltransferase responsible for forming both m7G2069 and m2G2445, the N-terminal RlmL activity for m2G2445 formation is significantly enhanced by the C-terminal RlmK. RlmKL has an unwinding activity of Helix 74, facilitating cooperative methylations of m7G2069 and m2G2445 during biogenesis of 50S subunit. For unwinding single-stranded RNA is a good substrate for RlmKL, substrate speccificity, overview. RlmKL is involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD | Escherichia coli |