Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + pseudouridine54 in tRNA | Haloferax volcanii | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | D4GTL8 | - |
- |
Methanocaldococcus jannaschii | Q59034 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mja_1640 methylates T-arm resembling tRNAs in vitro. Using synthetic 17 mer RNAs resembling the T-arm of Methanocaldococcus jannaschii tRNATrp that contains a pseudouridine at either position 54, 55, or both, the capability of MjNep1 to methylate these substrates is shown, MjNep1 methylates the substrate with a pseudouridine at position 55 with reasonable efficiency, but markedly slower than its native small ribosomal subunit rRNA-derived substrate, and no methylation is observed for the substrate with pseudouridine at position 54, MjNep1 is incapable of modifying mature H. volcanii tRNAs. MjNep1 is apparently not the pseudouridine-N1 methyltransferase responsible for the tRNA-modification at position 54 in archaeal tRNAs | Methanocaldococcus jannaschii | ? | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
COG 1901 | - |
Methanocaldococcus jannaschii |
Hvo_1989 | - |
Haloferax volcanii |
Mja_1640 | - |
Methanocaldococcus jannaschii |
Pfam DUF358 | - |
Haloferax volcanii |
Pfam DUF358 | - |
Methanocaldococcus jannaschii |
pseudouridine N1-specific methyltransferase | - |
Haloferax volcanii |
pseudouridine N1-specific methyltransferase | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
evolution | phylogeny of the methyltransferase family, overview | Haloferax volcanii |
evolution | the enzyme is a member of the SPOUT-class family of RNA methyltransferases, phylogeny of the methyltransferase family, overview | Methanocaldococcus jannaschii |
malfunction | a Hvo_1989 knockout strain lacks N1-methyl pseudouridine modifications at position 54 of its tRNAs, the in-frame deletion of the pseudouridine N1-methyltransferase gene in Haloferax volcanii does not result in a discernable phenotype | Haloferax volcanii |