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Literature summary for 2.1.1.257 extracted from

  • Wurm, J.; Griese, M.; Bahr, U.; Held, M.; Heckel, A.; Karas, M.; Soppa, J.; Woehnert, J.
    Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs (2012), RNA, 18, 412-420.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + pseudouridine54 in tRNA Haloferax volcanii
-
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
?
S-adenosyl-L-methionine + pseudouridine54 in tRNA Methanocaldococcus jannaschii
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S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GTL8
-
-
Methanocaldococcus jannaschii Q59034
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Mja_1640 methylates T-arm resembling tRNAs in vitro. Using synthetic 17 mer RNAs resembling the T-arm of Methanocaldococcus jannaschii tRNATrp that contains a pseudouridine at either position 54, 55, or both, the capability of MjNep1 to methylate these substrates is shown, MjNep1 methylates the substrate with a pseudouridine at position 55 with reasonable efficiency, but markedly slower than its native small ribosomal subunit rRNA-derived substrate, and no methylation is observed for the substrate with pseudouridine at position 54, MjNep1 is incapable of modifying mature H. volcanii tRNAs. MjNep1 is apparently not the pseudouridine-N1 methyltransferase responsible for the tRNA-modification at position 54 in archaeal tRNAs Methanocaldococcus jannaschii ?
-
?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
-
Haloferax volcanii S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
-
Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
?

Synonyms

Synonyms Comment Organism
COG 1901
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Methanocaldococcus jannaschii
Hvo_1989
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Haloferax volcanii
Mja_1640
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Methanocaldococcus jannaschii
Pfam DUF358
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Haloferax volcanii
Pfam DUF358
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Methanocaldococcus jannaschii
pseudouridine N1-specific methyltransferase
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Haloferax volcanii
pseudouridine N1-specific methyltransferase
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Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Methanocaldococcus jannaschii

General Information

General Information Comment Organism
evolution phylogeny of the methyltransferase family, overview Haloferax volcanii
evolution the enzyme is a member of the SPOUT-class family of RNA methyltransferases, phylogeny of the methyltransferase family, overview Methanocaldococcus jannaschii
malfunction a Hvo_1989 knockout strain lacks N1-methyl pseudouridine modifications at position 54 of its tRNAs, the in-frame deletion of the pseudouridine N1-methyltransferase gene in Haloferax volcanii does not result in a discernable phenotype Haloferax volcanii