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Literature summary for 2.1.1.255 extracted from

  • Kakimoto, M.; Ishikawa, T.; Miyagi, A.; Saito, K.; Miyazaki, M.; Asaeda, T.; Yamaguchi, M.; Uchimiya, H.; Kawai-Yamada, M.
    Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata (2014), J. Plant Physiol., 171, 292-300.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene pgmtf, real-time-RT PCR enzyme expression analysis Pseudanabaena galeata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + geranyl diphosphate Pseudanabaena galeata
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudanabaena galeata U6C870 gene pgmtf
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + geranyl diphosphate
-
 Pseudanabaena galeata S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
GPP methyltransferase
-
 Pseudanabaena galeata
GPPMT
-
 Pseudanabaena galeata

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Pseudanabaena galeata

Expression

Organism Comment Expression
Pseudanabaena galeata the expression level of the gene encoding GPPMT decreases at 4°C and increased at 30°C, compared with the level at 20°C down
Pseudanabaena galeata the expression level of the gene encoding GPPMT decreases at 4°C and increased at 30°C, compared with the level at 20°C up

General Information

General Information Comment Organism
metabolism some filamentous cyanobacteria produce 2-methylborneol via a two-step biosynthetic pathway: methylation of geranyl diphosphate (GPP) by methyl transferase (GPPMT), followed by the cyclization of methyl-GPP by monoterpene cyclase (MIBS). Pseudanabaena galeata, the filamentous cyanobacterium known to be a major causal organism of 2-methylborneol production in Japanese lakes. Metabolite analysis shows dramatic changes in primary metabolite concentrations in cyanobacteria grown at different temperatures. The data indicate that changes in carbon flow in the TCA cycle affect 2-MIB biosynthesis at higher temperatures Pseudanabaena galeata